Archives of Microbiology

, Volume 118, Issue 3, pp 253–256 | Cite as

Partial purification of a competence factor from Rhizobium japonicum

  • Abhaya M. Dandekar
  • Haren A. Vasavada
  • V. V. Modi
Article
  • 15 Downloads

Abstract

A competence factor (CF) from Rhizobium japonicum was partially purified to 43 fold on Sephadex G-100. This CF preparation was sensitive to heat, trypsin and pronase, was resistant to DNase 1, RNase A and lysozyme. It had an approximate mol. wt. of 82,000. Osmotic shock treatment of competent cells revealed that the CF is located in the periplasmic region of the cell.

Key words

Rhizobium japonicun Competence Competence factor Genetic transformation 

Abbreviations

CF

competence factor

BSA

bovine serum albumin

YM

yeast mannitol medium

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Akrigg, A., Ayad, S. R., Barker, G. R.: The nature of a competence-inducing factor in Bacillus subtillis. Biochem. Biophys. Res. Commun. 28, 1062–1067 (1967)Google Scholar
  2. Balassa, R.: Durch Desoxyribonukleinsäuren induzierte Veränderungen bei Rhizobien. Acta Microbiol. Acad. Sci. Hung. 4, 77–84 (1957a)Google Scholar
  3. Balassa, G.: Genetic transformation of Rhizobium. A review of the work of R. Balassa. Bacteriol. Rev. 27, 228–241 (1963)Google Scholar
  4. Balassa, R.: Einige Bedingungen der Ausbildung einer durch Deoxyribonukleinsäuren induzierten niedrigen Streptomycin-Resistenz bei Rhizobien. Acta Microbiol. Acad. Sci. Hung. 4, 85–95 (1957b)Google Scholar
  5. Balassa, R., Gabor, M.: Transformation of Rhizobium. Mikrobiologiya 30, 457–463 (1961)Google Scholar
  6. Charpak, M., Dedonder, R. M.: Production d'un facteur de competence soluble par Bacillus subtilis Marburg ind 168. C.R. Acad. Sci. 260, 5638–5640 (1965)Google Scholar
  7. Dandekar, A. M., Desai, A. J., Parikh, J. K., Vasavada, H. A., Modi, V. V.: Problems and genetic strategy for studies on Rhizobium japonicum. Proc. Dept. Atomic Energy Symp. on Genetics, Jan. 10–11, 1977, D. A. E. Govt. of India 208–222 (1977)Google Scholar
  8. Dobrzanski, W. T., Osowiecki, H.: Isolation and some properties of the competence factor from group H Streptococcus strain challis. J. Gen. Microbiol. 48, 299–304 (1967)Google Scholar
  9. Ellis, N. J., Kalz, G. G., Doncaster, J. J.: Transformation in Rhizobium trifolii. Can. J. Microbiol. 8, 835–840 (1962)Google Scholar
  10. Kohn, J.: A simple method for the concentration of fluids containing protein. Nature 183, 1055 (1959)Google Scholar
  11. Lowry, O. H., Rosenbrough, N. J., Farr, A. L., Randall, R. J.: Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193, 265–275 (1951)Google Scholar
  12. Neu, H. C., Heppel, L. A.: The release of enzymes from Escherichia coli by osmotic shock and during the formation of spheroplasts. J. Biol. Chem. 240, 3685–3692 (1965)Google Scholar
  13. Pakula, R., Walczak, W.: On the nature of competence of transformable Streptococci. J. Gen. Microbiol. 31, 125–133 (1963)Google Scholar
  14. Raina, J. L., Modi, V. V.: Genetic transformation in Rhizobium J. Gen. Microbiol. 57, 125–130 (1969)Google Scholar
  15. Raina, J. L., Modi, V. V.: Further studies on genetic transformation in Rhizobium. J. Gen. Microbiol. 65, 161–165 (1971)Google Scholar
  16. Raina, J. L., Modi, V. V.: Deoxyribonucleate binding and transformation in Rhizobium japonicum. J. Bacteriol. 111, 356–360 (1972)Google Scholar
  17. Tomasz, A.: Some aspects of the competent state in genetic transformation. Annu. Rev. Genet. 3, 217–232 (1969)Google Scholar
  18. Tomasz, A., Hotchkiss, R. D.: Regulation of the transformability of Pneumococcal cultures by Macromolecular cell products. Proc. Nat. Acad. Sci. U.S.A. 51, 480–487 (1964)Google Scholar
  19. Tomasz, A., Mosser, J. L.: On the nature of Pneumococcal activator substance. Proc. Nat. Acad. Sci. U.S.A. 55, 58–66 (1966)Google Scholar
  20. Vincent, J. M.: A mannual for the practical study of root module bacteria. IBP Handbook No. 15, pp. 3. Oxford-Edinburgh: Blackwell 1970Google Scholar
  21. Zelanza, I.: Transformation in Rhizobium trifolii. II. Development of competence. Acta Microbiol. Pol. 13, 291–298 (1964)Google Scholar
  22. Zoon, K. C., Haberstat, M., Scocca, J. J.: Synthesis of envelope polypeptides by Haemophilus influenzae during the development of competence for genetic transformation. J. Bacteriol. 127, 545–554 (1976)Google Scholar

Copyright information

© Springer-Verlag 1978

Authors and Affiliations

  • Abhaya M. Dandekar
    • 1
  • Haren A. Vasavada
    • 1
  • V. V. Modi
    • 1
  1. 1.Department of Microbiology, Faculty of ScienceM. S. University of BarodaBarodaIndia

Personalised recommendations