Archives of Microbiology

, Volume 118, Issue 3, pp 253–256 | Cite as

Partial purification of a competence factor from Rhizobium japonicum

  • Abhaya M. Dandekar
  • Haren A. Vasavada
  • V. V. Modi


A competence factor (CF) from Rhizobium japonicum was partially purified to 43 fold on Sephadex G-100. This CF preparation was sensitive to heat, trypsin and pronase, was resistant to DNase 1, RNase A and lysozyme. It had an approximate mol. wt. of 82,000. Osmotic shock treatment of competent cells revealed that the CF is located in the periplasmic region of the cell.

Key words

Rhizobium japonicun Competence Competence factor Genetic transformation 



competence factor


bovine serum albumin


yeast mannitol medium


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Copyright information

© Springer-Verlag 1978

Authors and Affiliations

  • Abhaya M. Dandekar
    • 1
  • Haren A. Vasavada
    • 1
  • V. V. Modi
    • 1
  1. 1.Department of Microbiology, Faculty of ScienceM. S. University of BarodaBarodaIndia

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