Archives of Microbiology

, Volume 108, Issue 2, pp 189–194 | Cite as

Synchronized cultures of a cell wall-less mutant of Chlamydomonas reinhardii

  • Torleiv Lien
  • Gjert Knutsen


Synchronization and synchronous growth of a cell wall-less mutant of Chlamydomonas reinhardii have been described. The following growth conditions were used: A modified Sueokas' “high salt minimal medium”, 14∶10 h light-dark cycle, growth temperature 30°C, light intensity 12–18 Klux and dilution of the culture at the end of the dark to a constant cell density of 1.0·106 cells/ml. The time course of increase and distribution of cell volume, cytoplasmic and nuclear division, release of motile cells after the division period and accumulation of DNA, RNA and protein are reported. These mutant cells did not make any sporangium in which the dividing cells were kept as a unit inside a mother cell wall. However, they usually adhered during the period of division, thus making clumps containing 2, 4 and 8 cells. Several of these cell clumps dissolved releasing either single or couples of 2 and 4 cells. After the end of division the cells became flagellated and motile and thereby releasing themselves from the aggregate.

Key words

Chlamydomonas reinhardii Synchronous growth Cell wall-less mutant 

Non-Standard Abbreviations


average weighed cell volume


minimal medium


high salt medium


trichloroacetic acid


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Copyright information

© Springer-Verlag 1976

Authors and Affiliations

  • Torleiv Lien
    • 1
    • 2
  • Gjert Knutsen
    • 1
    • 2
  1. 1.Institute for General MicrobiologyUniversity of BergenBergen-UniversitetetNorway
  2. 2.Botanical LaboratoryUniversity of BergenBergen-UniversitetetNorway

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