Summary
Microdissection and microcloning of the Y chromosomal lampbrush loop pseudonucleolus from primary spermatocytes of Drosophila hydei allowed the isolation of copies of a 0.2 kb tandemly repeated DNA sequence family (dhMiP14.1). In situ hybridization confirmed its origin from a distal position in the long arm of the Y chromosome. Transcript in situ hybridization showed that at least some of the repeat units are part of the transcripts of lampbrush loops. The characterization of this DNA sequence revealed cross-homology to the β2 region of the 26 S rRNA gene, 600 bp downstream of the intervening sequence (IVS) insertion site. The cloned DNA sequence, however, cannot be part of the rDNA repeats since it occurs as a 0.2 kb tandem repeat in the Y chromosome. We therefore named the DNA sequence the “rally” (ribosomal and lampbrush loop Y chromosome) sequence. The different repeat units of the 0.2 kb sequence display a moderate degree of divergence. They are not found in the closely related species D. neohydei or D. eohydei, nor in D. melanogaster. We assume that this Y chromosomal DNA sequence is the result of a recent transposition into the Y chromosomal location, followed by an amplification event. It is an important observation that the 0.2 kb sequence also occurs in the neighbouring lampbrush loop “threads”. Since other DNA sequences are also found in both loops, the assumption of a functional relationship between both loop pairs, derived from genetic observations, is further supported.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Akam M (1983) The location of Ultrabithorax transcripts in Drosophila tissue sections. EMBO J 2: 2075–2084
Amersham (1984) M13 cloning and sequencing handbook. Ame-sham International, Amersham, UK
Barnes SR, Webb DA, Dover G (1978) The distribution of satellite and main-band DNA components in the melanogaster species subgroup of Drosophila. I. Fractionation of DNA in Actinomycin D and Distamycin A density gradients. Chromosoma 67: 341–363
Barta A, Steiner G, Brosius J, Noller HF, Kuechler E (1984) Identification of a site on 23 S ribosomal RNA located at the peptidyl transferase center. Proc natl Acad Sci USA 81: 3607–3611
Brimacombe R, Stiege W, (1985) Structure and function of ribosomal RNA. Biochem J 229: 1–17
Chirgwin JM, Przybyla AE, MacDonald RJ, Rutter WJ (1979) Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry 18: 5294–5299
Clark CG, Tague BW, Ware VC, Gerbi SA (1984) Xenopus laevis 28 S ribosomal RNA: A secondary structure model and its evolution and functional implications. Nucleic Acids Res 12: 6197–6220
Denhardt DT (1966) A membrane-filter technique for the detection of complementary DNA. Biochem Biophys Res Commun 23: 641–646
Franz G, Tautz D, Dover G (1985) Conservation of major nuclease S1-sensitive sites in the non-conserved spacer region of ribosomal DNA in Drosophila species. J Mol Biol 183: 519–527
Grond CJ, Siegmund I, Hennig W (1983) Visualization of a lampbrush loop-forming fertility gene in Drosophila hydei. Chromosoma 88: 50–56
Hackstein JHP, Leoncini O, Beck H, Peelen G, Hennig W (1982) Genetic fine structure of the Y chromosome of Drosophila hydei. Genetics 101: 257–277
Hackstein JHP, Hennig W (1982) Mutants of Drosophila hydei. Dros Inf Serv 58: 195–203
Hennig I (1982) “Hybrid” X-Y translocation chromosomes of Drosophila hydei and neohydei. chromosoma 86: 491–508
Hennig W (1967) Untersuchungen zur Struktur und Funktion des Lampenbürsten-Y-Chromosoms in der Spermatogenese von Drosophila. Chromosoma 22: 294–357
Hennig W (1985) Y chromosome function and spermatogenesis in Drosophila hydei. Adv Genet 23: 179–234
Hennig W, Meyer GF, Hennig I, Leoncini O (1974) Structure and function of the Y chromosome of Drosophila hydei. Cold Spring Harbor Symp Quant Biol 38: 673–683
Hennig W, Vogt P, Jacob G, Siegmund I (1982) Nucleolus organizer regions in Drosophila species of the repleta group. Chromosoma 87: 279–292
Hennig W, Huijser P, Vogt P, Jäckle H, Edström J-E (1983) Molecular cloning of microdissected lampbrush loop DNA sequences of Drosophila hydei. EMBO J 2: 1741–1746
Hulsebos TJM, Hackstein JHP, Hennig W (1984) Lampbrush loop-specific protein of Drosophila hydei. Proc Natl Acad Sci USA 81: 3404–3408
Kremer H, Hennig W, Dijkhof R (1986) Chromatin organization in the male germ line of Drosophila hydei. Chromosoma 94: 147–161
Kunz W, Petersen G, Renkawitz-Pohl R, Glätzer KH, Schäfer M (1981) Distribution of spacer length classes and the intervening sequence among different nucleolus organizers in Drosophila hydei. Chromosoma 83: 145–158
Lifschytz E, Hareven D, Azriel A, Brodsly H (1983) DNA clones and RNA transcripts of four lampbrush loops from the Y chromosome of Drosophila hydei. Cell 32: 191–199
de Loos F, Dijkhof R, Grond CJ, Hennig W (1984) Lampbrush loop-specificity of transcript morphology in spermatocyte nuclei of Drosophila hydel. EMBO J 3: 2845–2849
Mandal RK, Dawid IB (1981) The nucleotide sequence at the transcription termination site of ribosomal RNA in Drosophila melanogaster. Nucleic Acids Res 9: 1801–1811
Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning. A laboratory manual. Cold Spring Harbor laboratory, New York
Messing J, Vieira J (1982) A new pair of M13 vectors for selecting either DNA strand of double-digest restriction fragments. Gene 19: 269–276
Renkawitz-Pohl R, Glätzer KH, Kunz W (1980) Characterization of cloned ribosomal DNA from Drosophila hydei. Nucleic Acids Res 8: 4593–4611
Roiha H, Glover DM (1981) Duplicated rDNA sequences of variable lengths flanking the short type I insertion in the rDNA of Drosophila melanogaster. Nucleic Acids Res 9: 5521–5532
Saedler H, Nevers P (1985) Transposition in plants: a molecular model. EMBO J 4: 585–590
Sanger F, Nicklen S, Coulson AR (1977) DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74: 5463–5467
Scalenghe F, Turco E, Edström J-E, Pirrotta V, Melli M (1981) Microdissection and cloning of DNA from a specific region of Drosophila melanogaster polytene chromosomes. Chromosoma 82: 205–216
Thomas PS, (1980) Hybridization of denatured RNA and small RNA fragments transferred to nitrocellulose. Proc Natl Acad Sci USA 77: 5201–5205
Vogt P, Hennig W (1983) Y chromosomal DNA of Drosophila hydei. J Mol Biol 167: 37–56
Vogt P, Hennig W (1986a) Molecular structure of the lampbrush loop nooses of the Y chromosome of Drosophila hydei. I. The Y chromosome-specific repetitive DNA sequence family ayl is dispersed in the loop DNA. Chromosoma 94: 449–458
Vogt P, Hennig W (1986b) Molecular structure of the lampbrush loop nooses of the Y chromosome of Drosophila hydei. II. DNA sequences with homologies to multiple genomic locations are major constituents of the loop. Chromosoma 94: 459–467
Vogt P, Hennig W, Siegmund I (1982) Identification of cloned Y chromosomal DNA sequences from a lampbrush loop of Drosophila hydei. Proc Natl Acad Sci USA 79: 5132–5136
Vogt P, Hennig W, Hacken D ten, Verbost P (1986) Evolution of Y chromosomal lampbrush loop DNA sequences of Drosophila. Chromosoma 94: 367–376
Ware VC, Tague BW, Clark CH, Gourse RL, Brand RC, Gerbi SA (1983) Sequence analysis of 38 S ribosomal DNA from the amphibian Xenopus laevis. Nucleic Acids Res 11: 7795–7817
Yamasaki N (1977) Selective staining of Y chromosomal loops in Drosophila hydei, D. neohydei and D. eohydei. Chromosoma 60: 27–37
Zacharias H, Hennig W, Leoncini O (1982) Comparative studies of Drosophila hydei and related species. Microspectrophotometry of genome sizes. Genetics 58: 153–157
Author information
Authors and Affiliations
Additional information
Communicated by J.A. Campos-Ortega
Rights and permissions
About this article
Cite this article
Huijser, P., Hennig, W. Ribosomal DNA-related sequences in a Y chromosomal lampbrush loop of Drosophila hydei . Mol Gen Genet 206, 441–451 (1987). https://doi.org/10.1007/BF00428884
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00428884