Molecular and General Genetics MGG

, Volume 206, Issue 3, pp 368–376 | Cite as

Regulation of the ban gene containing operon of prophage P1

  • Anke Heisig
  • Iris Severin
  • Ann-Kristin Seefluth
  • Heinz Schuster
Article

Summary

A physical map of the ban gene of P1 and sites relevant to its regulation has been deduced from cloning of the appropriate regions of P1 wild-type and of P1 ban regulatory mutants. The cloning required the presence of P1 repressor in the cell confirming the existence of a repressible ban operon (Austin et al. 1978). Evidence for additional member(s) of that operon is presented. Of particular interest for understanding the regulation of ban are the relative positions of a binding site for the P1 repressor and of the regulatory mutations bac and crr that render ban expression constitutive. The results reveal a repressible operon-like structure of about 4 kb within the P1 EcoRI-3 fragment that comprises a c1 repressor binding site/bac additional gene(s) — crr/ban in the clockwise direction of the circular map of P1.

Key words

P1 repressor Repressible promoter ban regulatory mutants Gene cloning 

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Copyright information

© Springer-Verlag 1987

Authors and Affiliations

  • Anke Heisig
    • 1
  • Iris Severin
    • 1
  • Ann-Kristin Seefluth
    • 1
  • Heinz Schuster
    • 1
  1. 1.Max-Planck-Institut für Molekulare GenetikBerlin 33Germany

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