Archives of Microbiology

, Volume 141, Issue 4, pp 348–352 | Cite as

Energy metabolism in Streptococcus cremoris during lactose starvation

  • Roel Otto
  • John Vije
  • Bart ten Brink
  • Bert Klont
  • Wil N. Konings
Original Papers


The ATP pool of Streptococcus cremoris in a lactose-limited chemostat depletes rapidly when lactose is consumed. The decrease of the intracellular ATP concentration parallels the dissipation of the electrochemical proton gradient. The adenylate energy charge of growing cells is 0.8 but drops rapidly to 0.2 when the cells enter the starvation phase.

One of the early events of lactose starvation is a rapid increase of the pools of phosphoenolpyruvate and inorganic phosphate. The accumulation of phosphoenolpyruvate is temporarily and levels off at a much lower value than in growing cells; the accumulation of phosphate is of a more permanent nature. Despite the low PEP concentration starved cells are, after 24 h of incubation in the absence of lactose, still able to take up lactose, to synthesize ATP and to generate quickly an electrochemical proton gradient.

Key words

ATP Proton motive force Adenylate Energy charge Streptococcus cremoris 





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Copyright information

© Springer-Verlag 1985

Authors and Affiliations

  • Roel Otto
    • 1
  • John Vije
    • 1
  • Bart ten Brink
    • 1
  • Bert Klont
    • 1
  • Wil N. Konings
    • 1
  1. 1.Department of MicrobiologyUniversity of GroningenHarenThe Netherlands

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