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Archives of Microbiology

, Volume 141, Issue 4, pp 284–289 | Cite as

Structural analyses of lipid A from lipopolysaccharides of nodulating and non-nodulating Rhizobium trifolii

  • Ryszard Russa
  • Otto Lüderitz
  • Ernst Th. Rietschel
Original Papers

Abstract

Structural studies have been performed on lipid A preparations derived from lipopolysaccharides of nodulating (nod+) Rhizobium trifolii wild type strains and non-nodulating (nod-) mutants deprived of the medium size “Nod” plasmid.

All preparations contain a lipid A backbone composed of a β1,6-linked d-glucosamine disaccharide (GlcN II-β1,6-GlcN I) which is bis-phosphorylated at positions 1 and 4′. The phosphate group at position 4′ (GlcN II) is nonstoichiometrically substituted probably by a neutral glycosyl residue. Another substituent (probably also a neutral sugar) substitutes partly position 4 (GlcN I) of the disaccharide. The hydroxyl groups at positions 3 and 3′ are likely to be esterified by fatty acyl residue. In lipid A of nod+ strains, 3-hydroxyhexa- and octadecanoic acid are linked to the amino group of GlcN I, and 3-hydroxyhexa- and tetradecanoic acid to the amino group of GlcN II. In lipid A of nod- strains, mainly 3-hydroxyhexadecanoic acid is linked to the amino group of GlcN I, and 3-hydroxytetra- and hexadecanoic acid to that of GlcN II.

The results show that rhizobial lipid A expresses many similarities to the lipid A of Enterobacteria. The structure shows, however, also peculiarities, especially regarding substituents of the lipid A backbone.

Key words

Rhizobium trifolii Lipopolysaccharide Lipid A-structure “Nod” plasmids 3-Hydroxy fatty acids 

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Copyright information

© Springer-Verlag 1985

Authors and Affiliations

  • Ryszard Russa
    • 3
  • Otto Lüderitz
    • 1
  • Ernst Th. Rietschel
    • 2
  1. 1.Max-Planck-Institut für ImmunbiologieFreiburgFederal Republic of Germany
  2. 2.Forschungsinstitut BorstelBorstelFederal Republic of Germany
  3. 3.Institute of MicrobiologyM. Curie-Sklodowska UniversityLublinPoland

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