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Asymmetric repair of bacteriophage T7 heteroduplex DNA

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Summary

Heteroduplex DNA molecules were prepared in vitro using one strand of DNA carrying a point mutation and one strand of the corresponding wild-type DNA. The heteroduplex DNA was transfected into competent bacteria and the progeny genotypes in the resulting infective centers were determined. From the results were conclude that about 80% of all transfected DNA molecules are repaired before DNA replication starts. This fraction of repaired DNA is independent of the location of the mismatched nucleotide pair. However, mismatch correction occurs preferentially on the H strand of the heteroduplex DNA.

The repair does not depend on a known phage coded function but requires the active bacterial genes mut U, mut H, mut S and probably mut L.

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Author notes

  1. Joachim Bauer

    Present address: Institut für Biochemie, Fabeckstr. 33-34, D-1000, Berlin 33

Authors and Affiliations

  1. Fakultät für Biologie, Universität Konstanz, Postfach 5560, D-7750, Konstanz, Federal Republic of Germany

    Joachim Bauer, Günter Krämmer & Rolf Knippers

Authors
  1. Joachim Bauer
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  2. Günter Krämmer
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  3. Rolf Knippers
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Communicated by H. Saedler

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Bauer, J., Krämmer, G. & Knippers, R. Asymmetric repair of bacteriophage T7 heteroduplex DNA. Molec. Gen. Genet. 181, 541–547 (1981). https://doi.org/10.1007/BF00428750

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  • DOI: https://doi.org/10.1007/BF00428750

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