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Introduction of active enzymes into intact Escherichia coli cells by means of liposomes

Phenotypic suppression of uvr A and pol A mutants

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Summary

Genetically deficient cells were supplied with the missing enzymes, purified from an independent source. The introduction of exogenous enzymes into the cells was effected by two independent methods: plasmolysis and liposome transformation. The latter procedure yielded a homogenous cell population which had been rescued from the defect even if the molecular weight of the enzyme amounted to 70 KD (Kilodaltons).

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References

  • Abramson D, Blecher M (1964) Quantitative two-dimensional thinlayer chromatography of naturally occurring phospholipids. J Lipid Res 5:628–631

    Google Scholar 

  • Bekker M, Kaboev O, Akhmedov A, Luchkina L (1980) Ultra-violetendonuclease activity in cell extracts of Sacharamyces cerevisia mutants defective in excision of pyrimidine dimers. J Bacteriol 142:322–324

    Google Scholar 

  • Bresler S, Noskin L, Stepanova I, Kusovleva N (1978) Mechanism of the radioprotecting action of chemical compounds on Escherichia coli cells. Mol gen Genet 163:75–85

    Google Scholar 

  • Chong C, Colbow K (1976) Light scattering and turbidity mesearements on lipid vesicles. Biochim Biophys Acta 436:260–282

    Google Scholar 

  • Chu B (1974) Laser light scattering. Academic Press, New York

    Google Scholar 

  • Cohen S, Chang A, Hsu L (1972) Nonchromosomal antibiotic resistance in bacteria: Genetic transformation of Escherichia coli by R-factor DNA. Proc Natl Acad Sci USA 69:2110–2114

    Google Scholar 

  • Folch J, Lees M, Sloane-Stanley M (1957) J Biol Chem 226:497–502

    Google Scholar 

  • Hanahan D, Turner M, Sayko (1951) The isolation of egg phosphatidylcholine by an absorbtion columne technique. J Biol Chem 192:623–630

    Google Scholar 

  • Kaboev O, Luchkina L, Akhmedov A, Bekker, M (1981) Purification and properties of deoxyribonucleic acids polymerase I from Bacillus stearothermophilus. J Bacteriol 145:21–26

    Google Scholar 

  • Pagano R, Weinstein J (1978) Liposomes. Annu Rev Biophys Bioeng 7:435–468

    Google Scholar 

  • Post G, Papahadjopulos D (1976) Lipid vesicles as carriers for introducing matherials into cultured cells: Influence of vesicle lipid composition on mechanism of vesicle incorporation into cells. Proc Natl Acad Sci 73:5, 1603–1607

    Google Scholar 

  • Shimizu K, Sekiguchi M (1979) Introduction of an active enzyme into cells of Escherichia coli, Mol Gen Genet 168:37–47

    Google Scholar 

  • Singleton WS, Gray MS, Brown ML, White S (1965) Chromatographycally homogenous lecithin from egg phospholipids. J Am Oil Chem Soc 42:53–56

    Google Scholar 

  • Szoka F, Papahadjopoulos D (1978) Procedure for preparation of liposomes with large internal aqueous space and high capture by reversephase evaporation. Proc Natl Acad Sci USA 75:9, 4194–4198

    Google Scholar 

  • Vaskovsky VE, Kostetsky E, Vasendin I (1975) J Chromatography 114:129–134

    Google Scholar 

  • Yasuda S, Sekiguchi M (1976) Futher purification and characterisation of T4 endonuclease Y. Biochim Biophys Acta 442:197–207

    Google Scholar 

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Author notes

  1. E. F. Davidenkova, O. A. Rosenberg & E. I. Schwartz

    Present address: Research Division of Prof. E.F. Davidenkova, Leningrad, USSR

Authors and Affiliations

  1. Leningrad Institute of Nuclear Physics, Academy of Sciences of the USSR, Gatchina, Leningrad, USSR

    S. E. Bresler, L. A. Noskin, O. K. Kaboev, I. M. Stepanova, E. F. Davidenkova, O. A. Rosenberg, E. I. Schwartz, A. G. Beketova & A. T. Akhmedov

Authors
  1. S. E. Bresler
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  2. L. A. Noskin
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  3. O. K. Kaboev
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  4. I. M. Stepanova
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  5. E. F. Davidenkova
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  6. O. A. Rosenberg
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  7. E. I. Schwartz
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  8. A. G. Beketova
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  9. A. T. Akhmedov
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Communicated by D. Goldfarb

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Bresler, S.E., Noskin, L.A., Kaboev, O.K. et al. Introduction of active enzymes into intact Escherichia coli cells by means of liposomes. Molec. Gen. Genet. 181, 532–534 (1981). https://doi.org/10.1007/BF00428748

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  • DOI: https://doi.org/10.1007/BF00428748

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