Molecular and General Genetics MGG

, Volume 181, Issue 4, pp 464–469 | Cite as

Physical and functional mapping of Tn2603, a transposon encoding ampicillin, streptomycin, sulfonamide, and mercury resistance

  • Tomoko Yamamoto
  • Michiyasu Tanaka
  • Rieko Baba
  • Saburo Yamagishi
Article

Summary

A map of cleavage sites for restriction endonucleases EcoR1, BamHI, HindIII, and SalI on Tn2603, a transposon encoding resistance to ampicillin, streptomycin, sulfonamide, and mercury, was constructed by an analysis of restriction cleavage patterns of plasmid pMK1.:: Tn2603 and its deletion derivative. By cloning the fragments generated from pMK1::Tn2603 with these restriction endonucleases to a pACYC184 plasmid vehicle, the regions necessary for expression of resistance were located on the restriction cleavage map of Tn2603. Ampicillin, streptomycin, and sulfonamide-resistance genes were mapped in a cluster on the region between the center and the right and the mercury-resistance gene was located to the left of the map. The final functional map of Tn2603 was compared with those of Tn4 and Tn21 and the evolutional relationships between them were discussed.

Keywords

Sulfonamide Mercury Streptomycin Restriction Endonuclease Ampicillin 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Barth PT, Datta N, Hedges RW, Grinter JN (1976) Transposition of a deoxyribonucleic acid sequence encoding trimethoprim and streptomycin resistance from R483 to other replicons. J Bacteriol 125:800–810Google Scholar
  2. Barton CR, Warren RL, Jezo P, Easton AM, Rownd RH (1979) SalI restriction endonuclease maps of FII incompatibility group R plasmids. Plasmid 2:150–154Google Scholar
  3. Blohm D, Goebel W (1978) Restriction map of the antibiotic resistance plasmid Rldrd-19 and its derivatives pKN102 (Rldrd-19B2) and Rldrd-16 for the enzymes BamHI, HindIII, EcoRI and SalI. Mol Gen Genet 167:119–127Google Scholar
  4. Chandler M, Silver L, Lane D, Caro L (1979) Properties of an autonomous r-determinant from R100.1. Symposia on quantitative biology XLIII, 1223–1231Google Scholar
  5. Chang ACY, Cohen SN (1978) Construction and characterization of of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid. J. Bacteriol 134:1141–1156Google Scholar
  6. Cohen SN (1976) Transposable genetic elements and plasmid evolution. Nature 263:731–738Google Scholar
  7. Cohen, SN, Chang ACY, Hsu L (1972) Nonchromosomal antibiotic resistance in bacteria: Genetic transformation of Escherichia coli by R-factor DNA. Proc Natl Acad Sci USA 69:2110–2114Google Scholar
  8. Dale JW, Smith JT (1974) R-factor-mediated β-lactamases that hydrolyze oxacillin: evidence for two distinct groups. J Bacteriol 119:351–356Google Scholar
  9. Foster TJ, Nakahara H (1979) Deletions in the r-determinant mer region of plasmid R100-1 selected for loss of mercury hypersensitivity. J Bacteriol 140:301–305Google Scholar
  10. Hedges RW, Datta N, Coetzee JN (1973) R factors from Proteus morganii. J Gen Microbiol 77:249–259Google Scholar
  11. Hedges RW, Datta N, Kontomichalou P, Smith JT (1974) Molecular specificities of R-factor-determined β-lactamase: correlation with plasmid compatibility. J Bacteriol 117:56–62Google Scholar
  12. Jacob AE, Shapiro JA, Yamamoto L, Smith DI, Cohen SN, Berg D (1977) Bacterial plasmids: plasmids studied in Escherichia coli and other bacterial In: Bukhari AI, Shapiro JA, Adhya SL (eds) DNA insertion elements, plasmids, and episomes. Cold Spring Harbor Laboratory, New York, pp 607–704Google Scholar
  13. Kleckner N (1977) Translocatable elements in procaryotes. Cell 11:11–23Google Scholar
  14. Kopecko DJ, Brevet J, Cohen SN (1976) Involvement of multiple translocating DNA segments and recombinational hot spots in the structural evolution of bacterial plasmids. J Mol Biol 108:333–360Google Scholar
  15. Kopecko DJ, Cohen SN (1975) Site-specific recA-independent recombination between bacterial plasmids: involvement of palindromes at the recombinational loci. Proc Natl Acad Sci USA 72:1373–1377Google Scholar
  16. Maniatis T, Jeffrey A, Van deSande H (1975) Chain length determination of small double and single-stranded DNA molecules by polyacrylamide gel electrophoresis. Biochemistry 14:3787–3794Google Scholar
  17. Nisen PD, Kopecko DJ, Chou J, Cohen SN (1977) Site-specific deletions occurring adjacent to the termini of transposable ampicillin resistance element (Tn3). J Mol Biol. 117:975–998Google Scholar
  18. Robert CA, Yang JL, Wu R (1979) Elution of DNA from agarose gels after electrophoresis. Method in Enzymol 68:176–182Google Scholar
  19. Yamagishi S, O'hara K, Sawai T, Mitsuhashi S (1969) The purification and properties of penicillin β-lactamases mediated by transmissible R factor in Escherichia coli. J Biochem 66:11–20Google Scholar
  20. Yamamoto T, Katoh R, Shimazu A, Yamagishi S (1980) Gene expression of ampicillin resistance transposons, Tn2601 and Tn2602. Microbiol. Immunol 24:479–494Google Scholar
  21. Yamamoto T, Tanaka M, Nohara C, Fukunaga Y, Yamagishi S (1981) Transposition of oxacillin-hydrolysing penicillinase gene. J Bacteriol 145:808–813Google Scholar
  22. Yamamoto T, Yokota T (1980) Construction of a physical map of a kanamycin (Km) transposons, Tn5, and a comparison to another Km transposon, Tn903. Mol Gen Genet 178:79–83Google Scholar

Copyright information

© Springer-Verlag 1981

Authors and Affiliations

  • Tomoko Yamamoto
    • 1
  • Michiyasu Tanaka
    • 1
  • Rieko Baba
    • 1
  • Saburo Yamagishi
    • 1
  1. 1.Division of Microbial Chemistry, Faculty of Pharmaceutical SciencesChiba UniversityChibaJapan

Personalised recommendations