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Utilization of nitrogen compounds and ammonia assimilation by chromatiaceae

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Abstract

Chromatium vinosum strain D, Thiocapsa roseopersicina strain 6311 and Ectothiorhodospira mobilis strain 8112 were grown anaerobically in the light with various single nitrogen sources. When substituted for NH4Cl only glutamine and casamino acids supported good growth of all strains tested. Peptone and urea were utilized by C. vinosum and T. roseopersicina, glutamate, asparagine and nitrate only by C. vinosum. The strains were able to grow with molecular nitrogen; complete inhibition of this growth was observed in the presence of alanine with E. mobilis, and of alanine or asparagine with T. roseopersicina.

Glutamate dehydrogenase, requiring either NADH or NADPH, NADH-linked glutamate synthase, and glutamine synthetase were demonstrate in the above organisms grown on NH4Cl.

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References

  • Beisenherz, G., Boltze, H. J., Bücher, T., Czok, R., Garbade, K. H., Meyer-Arendt, E., Pfleiderer, G.: Diphosphofructose-Aldolase, Phosphoglyceraldehyd-Dehydrogenase, Milchsäure-Dehydrogenase, Glycerophosphat-Dehydrogenase und Pyruvat-Kinase aus Kaninchenmuskulatur in einem Arbeitsgang. Z. Naturforsch. 8b, 555–577 (1953)

    Google Scholar 

  • Dalton, H.: Fixation of dinitrogen by free-living microorganisms. CRC Crit. Rev. Microbiol. 3, 183–220 (1974)

    Google Scholar 

  • Elmerich, C.: Le cycle du glutamate, point de départ du métabolisme de l'azote, chez Bacillus megaterium. Europ. J. Biochem. 27, 216–224 (1972)

    Google Scholar 

  • Gogotov, I. N., Glinsky, V. P.: Comparative study of nitrogen faxation in purple bacteria. Mikrobiologiya 42, 983–986 (1973)

    Google Scholar 

  • Hurlbert, R. E., Lascelles, J.: Ribulose diphosphate carboxylase in Thiorhodaceae. J. gen. Microbiol. 33, 445–458 (1963)

    Google Scholar 

  • Kondratieva, E. N., Malofeeva, I. V., Sumarokova, R. S.: An ability for reductive amination and transamination in Chromatium minutissimum. Mikrobiologiya 38, 13–17 (1969)

    Google Scholar 

  • Malofeeva, I. V., Bogorov, L. V., Gogotov, I. N.: Assimilation of nitrates by purple bacteria. Mikrobiologiya 43, 967–972 (1974)

    Google Scholar 

  • Matheron, R., Baulaigue, R.: Bacteries photosynthétiques sulfureuses marines. Assimilation des substances organiques et minérales, et influence de la teneur en chlorure de sodium du milieu de culture sur leur développement. Arch. Mikrobiol. 86, 291–304 (1972)

    Google Scholar 

  • Mecke, D.: Methoden zur Bestimmung von Metaboliten: l-Glutamin. Bestimmung mit Glutamin-Synthetase. In: Methoden der enzymatischen Analyse, 2. Aufl., Bd. 2 (H. U. Bergmeyer, Hrsg.), S. 1667–1670. Weinheim: Verlag Chemie 1970

    Google Scholar 

  • Meister, A.: Glutaminase, asparaginase, and α-keto acid-ω-amidase. In: Methods in enzymology, Vol. 2 (S. P. Colowick, N. O. Kaplan, eds.), pp. 380–385. New York: Academic Press 1955

    Google Scholar 

  • Miller, R. E., Stadtman, E. R.: Glutamate synthase from Escherichia coli. An iron-sulfide flavoprotein. J. biol. Chem. 247, 7407–7419 (1972)

    Google Scholar 

  • Nagatani, H., Shimizu, M., Valentine, R. C.: The mechanism of ammonia assimilation in nitrogen fixing bacteria. Arch. Mikrobiol. 79, 164–175 (1971)

    Google Scholar 

  • Van Niel, C. B.: Techniques for the enrichment, isolation, and maintenance of the photosynthetic bacteria. In: Methods in enzymology, Vol. 23 (S. P. Colowick, N. O. Kaplan, eds.), pp. 3–28, New York-London: Academic Press 1971

    Google Scholar 

  • Osnitskaya, L. K.: The use of organic compounds by the photosynthesizing purple bacterium from the Beloie lake. Mikrobiologiya 27, 665–672 (1958)

    Google Scholar 

  • Petrova, E. A.: Sources of nitrogen nutrition for purple sulfur bacteria. Dokl. Akad. Nauk SSSR 126, 1100–1102 (1959)

    Google Scholar 

  • Pfennig, N., Trüper, H. G.: The phototrophic bacteria, In: Bergey's manual of determinative bacteriology, 8th ed. (R. E. Buchanan, N. E. Gibbons, eds.), pp. 24–64. Baltimore: Williams & Wilkins 1974

    Google Scholar 

  • Ravel, J. M.: Asparagine synthetase (Lactobacillus arabinosus). In: Methods in enzymology, Vol. 17A (S. P. Colowick, N. O. Kaplan, eds.), pp. 722–726, New York-London: Academic Press 1970

    Google Scholar 

  • Shapiro, B. M., Stadtman, E. R.: The regulation of glutamine synthesis in microorganisms. Ann. Rev. Microbiol. 24, 501–524 (1970a)

    Google Scholar 

  • Shapiro, B. M., Stadtman, E. R.: Glutamine synthetase (Escherichia coli). In: Methods in enzymology, Vol. 17A (S. P. Colowick, N. O. Kaplan, eds.), pp. 910–922. New York-London: Academic Press 1970b)

    Google Scholar 

  • Stewart, W. D. P.: Nitrogen fixation by photosynthetic microorganisms. Ann. Rev. Microbiol. 27, 283–316 (1973)

    Google Scholar 

  • Tempest, D. W., Meers, J. L., Brown, C. M.: Synthesis of glutamate in Aerobacter aerogenes by a hitherto unknown route. Biochem. J. 117, 405–407 (1970)

    Google Scholar 

  • Thiele, H. H.: Die Verwertung einfacher organischer Substrate durch Thiorhodaceae. Arch. Mikrobiol. 60, 124–138 (1968)

    Google Scholar 

  • Trüper, H. G.: CO2-Fixierung und Intermediärstoffwechsel bei Chromatium okenii Perty. Arch. Mikrobiol. 49, 23–50 (1964)

    Google Scholar 

  • Trüper, H. G.: Culture and isolation of phototrophic sulfur bacteria from the marine environment. Helgoländer wiss. Meeresunters. 20, 6–16 (1970)

    Google Scholar 

  • Wagner, B. J., Miović, M. L., Gibson, J.: Utilization of amino acids by Chromatium sp. strain D. Arch. Mikrobiol. 91, 255–272 (1973)

    Google Scholar 

  • Williams, V. R., Lartigue, D. J.: Aspartase. In: Methods in enzymology, Vol. 13 (S. P. Colowick, N. O. Kaplan, eds.), pp. 354–361. New York-London: Academic Press 1969

    Google Scholar 

  • Wu, C., Yuan, L. H.: Regulation of synthesis of glutamine synthetase in Escherichia coli. J. gen. Microbiol. 51, 57–65 (1968)

    Google Scholar 

  • Yang Hui-Fang: Morphological and physiological features of various strains of purple sulfur bacteria. Nauchn. Dokl. Vysshei Shkoly, Biol. Nauki 3, 163–169 (1962)

    Google Scholar 

  • Zakhvataeva, N. V., Malofeeva, I. V., Kondratieva, E. N.: A study on nitrogen fixation by photosynthetizing bacteria. Mikrobiologiya 39, 761–766 (1970)

    Google Scholar 

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Bast, E. Utilization of nitrogen compounds and ammonia assimilation by chromatiaceae. Arch. Microbiol. 113, 91–94 (1977). https://doi.org/10.1007/BF00428586

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