Archives of Microbiology

, Volume 113, Issue 1–2, pp 61–64 | Cite as

Sophorose metabolism and cellulase induction in Trichoderma

  • J. R. Loewenberg
  • C. M. Chapman


The cellulase inducer sophorose was rapidly catabolized to CO2 and H2O by Trichoderma: only small amounts were used to induce the synthesis of cellulase. 3H-sophorose uptake began after a lag of 1 h and its half-life in the medium was less than 5 h. Cellulase activity in the medium did not increase till 6 h after the addition of sophorose and reached a half maximum value at 14 h. The presence of free sophorose in the medium was required for continuous cellulase production. Several small sophorose addition induced much more cellulase than an equivalent single dose. These results are attributed to two pathways of sophorose utilization, a catabolic pathway that has a high capacity but low affinity for sophorose and an inductive pathway having a lower capacity but higher affinity for sophorose.

Key words

Cellulase Metabolism Sophorose Trichoderma 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Chapman, C. M., Loewenberg, J. R.: Ultracytochemistry of cellulase synthesis in the fungus Trichoderma. Ann. Mtg. Suppl. Plant Physiol. 57, 70 (1976)Google Scholar
  2. Child, J. J., Eveleigh, D. E., Sieben, A. S.: Determination of cellulase activity using hydroxyethylcellulose as a substrate. Canad. J. Biochem. 51, 39–43 (1973)Google Scholar
  3. Coxon, B., Fletcher, H. C.: Simplified preparation of sophorose (2-O-β-d-glucopyranosyl-d-glucose). J. Org. Chem. 26, 2892–2894 (1961)Google Scholar
  4. Mandels, M., Parrish, F. W., Reese, E. T.: Sophorose as an inducer of cellulase in Trichoderma viride. J. Bact. 83, 400–408 (1962)Google Scholar
  5. Mandels, M., Sternberg, D., Andreotti, R. E.: Growth and cellulase production by Trichoderma. In: Symposium on enzymatic hydrolysis of cellulose (M. Bailey, T. M. Enari, M. Linko, eds.), pp. 81–109. Helsinki: The Finnish National Fund for Research and Development (SITRA) 1975Google Scholar
  6. Nisizawa, T., Suzuki, H., Nakayama, M., Nisizawa, K.: Inductive formation of cellulase in Trichoderma viride. J. Biochem. 70, 375–385 (1971a)Google Scholar
  7. Nisizawa, T., Suzuki, H., Nisizawa, K.: De novo synthesis of cellulase induced by sophorose in Trichoderma viride cells. J. Biochem. 70, 387–393 (1971b)Google Scholar
  8. Nisizawa, T., Suzuki, H., Nisizawa, K.: Catabolite repression of cellulase formation in Trichoderma viride. J. Biochem. 71, 999–1007 (1972)Google Scholar
  9. Pollock, M. R.: Induced formation of enzymes. In: The enzymes, 2nd ed., Vol. 1 (P. D. Boyer, H. Lardy, K. Myrbäck, eds.), pp. 620–680. New York: Academic Press 1959Google Scholar
  10. Randerath, K.: An improved procedure for solid scintillation fluorography of tritium labeled compounds. Analyt. Chem. 41, 991–992 (1969)Google Scholar
  11. Trevelyan, W., Trevelyan, E., Procter, D. P., Harrison, J. S.: Detection of sugars on paper chromatograms. Nature (Lond.) 166, 444–445 (1950)Google Scholar

Copyright information

© Springer-Verlag 1977

Authors and Affiliations

  • J. R. Loewenberg
    • 1
  • C. M. Chapman
    • 1
  1. 1.Department of BotanyUniversity of Wisconsin-MilwaukeeMilwaukeeUSA

Personalised recommendations