Abstract
The cellulase inducer sophorose was rapidly catabolized to CO2 and H2O by Trichoderma: only small amounts were used to induce the synthesis of cellulase. 3H-sophorose uptake began after a lag of 1 h and its half-life in the medium was less than 5 h. Cellulase activity in the medium did not increase till 6 h after the addition of sophorose and reached a half maximum value at 14 h. The presence of free sophorose in the medium was required for continuous cellulase production. Several small sophorose addition induced much more cellulase than an equivalent single dose. These results are attributed to two pathways of sophorose utilization, a catabolic pathway that has a high capacity but low affinity for sophorose and an inductive pathway having a lower capacity but higher affinity for sophorose.
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References
Chapman, C. M., Loewenberg, J. R.: Ultracytochemistry of cellulase synthesis in the fungus Trichoderma. Ann. Mtg. Suppl. Plant Physiol. 57, 70 (1976)
Child, J. J., Eveleigh, D. E., Sieben, A. S.: Determination of cellulase activity using hydroxyethylcellulose as a substrate. Canad. J. Biochem. 51, 39–43 (1973)
Coxon, B., Fletcher, H. C.: Simplified preparation of sophorose (2-O-β-d-glucopyranosyl-d-glucose). J. Org. Chem. 26, 2892–2894 (1961)
Mandels, M., Parrish, F. W., Reese, E. T.: Sophorose as an inducer of cellulase in Trichoderma viride. J. Bact. 83, 400–408 (1962)
Mandels, M., Sternberg, D., Andreotti, R. E.: Growth and cellulase production by Trichoderma. In: Symposium on enzymatic hydrolysis of cellulose (M. Bailey, T. M. Enari, M. Linko, eds.), pp. 81–109. Helsinki: The Finnish National Fund for Research and Development (SITRA) 1975
Nisizawa, T., Suzuki, H., Nakayama, M., Nisizawa, K.: Inductive formation of cellulase in Trichoderma viride. J. Biochem. 70, 375–385 (1971a)
Nisizawa, T., Suzuki, H., Nisizawa, K.: De novo synthesis of cellulase induced by sophorose in Trichoderma viride cells. J. Biochem. 70, 387–393 (1971b)
Nisizawa, T., Suzuki, H., Nisizawa, K.: Catabolite repression of cellulase formation in Trichoderma viride. J. Biochem. 71, 999–1007 (1972)
Pollock, M. R.: Induced formation of enzymes. In: The enzymes, 2nd ed., Vol. 1 (P. D. Boyer, H. Lardy, K. Myrbäck, eds.), pp. 620–680. New York: Academic Press 1959
Randerath, K.: An improved procedure for solid scintillation fluorography of tritium labeled compounds. Analyt. Chem. 41, 991–992 (1969)
Trevelyan, W., Trevelyan, E., Procter, D. P., Harrison, J. S.: Detection of sugars on paper chromatograms. Nature (Lond.) 166, 444–445 (1950)
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Loewenberg, J.R., Chapman, C.M. Sophorose metabolism and cellulase induction in Trichoderma . Arch. Microbiol. 113, 61–64 (1977). https://doi.org/10.1007/BF00428581
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DOI: https://doi.org/10.1007/BF00428581