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Untersuchungen über das Transportsystem für Thiamin bei Bacillus cereus

Studies on the thiamine transport system in Bacillus cereus

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Abstract

The thiamine transport system in Bacillus cereus exhibits rhythmical changes of resorption-and excretion-phases lasting 1–2 h. These main phases are subdivided in shorter ones with an average duration of 45 s. The velocity of the thiamine uptake is influenced by pH, temperature, age of cells, energy and substrate supply and thiamine concentration of the medium. The Michaelis-Menten-Kinetic can be used to describe the uptake: K m =1.98x10-8 M; V max=1.19x10-6 mol/g dry weightxmin. The rate is enhanced by K+, Ca2+ and Mg2+, and inhibited by Pyrithiamin, EDTA, H+-ions, proton donors and proton acceptors; OH--ions cause a change in the direction of transport. A theoretical explanation can be given by assuming a coupling of the thiamine permeation with proton movements in the membrane.

Zusammenfassung

Bei dem untersuchten Transportsystem für Thiamin bei Bacillus cereus wechseln rhythmisch 1–2 h andauernde Aufnahme-und Abgabephasen miteinander ab. Diese großen Phasen sind in kleinere von durchschnittlich 45 s Dauer unterteilt. Die Geschwindigkeit der Thiaminaufnahme wird von pH-Wert, Temperatur, Alter der Zellen, Energie-und Nährstoffversorgung sowie Thiaminkonzentration des Mediums beeinflußt. Sie folgt der Michaelis-Menten-Kinetik: K m =1,98x10-8 M; V max=1,19x10-6 mol/g TGxmin. Gefördert wird die Aufnahmerate durch K+, Ca2+ und Mg2+, gehemmt wird sie durch Pyrithiamin, EDTA, H+-Ionen, Wasserstoffacceptoren und-donatoren; OH--Ionen bewirken eine Umkehr der Transportrichtung. Als erklärende Theorie wird eine Kopplung der Thiaminpermeation mit Protonenverschiebungen in der Membran diskutiert.

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Abbreviations

AMP:

Adenosinmonophosphat

ADP:

Adenosindiphosphat

ATP:

Adenosintriphosphat

DNP:

Dinitrophenol

EDTA:

Athylendiamintetraessigsäure

FAD:

Flavinadenindinucleotid

NAD:

Nicotinamidadenindinucleotid

NADP:

Nicotinamidadenindinucleotidphosphat

TPP:

Thiaminpyrophosphat

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  1. Botanisches Institut der Universität, Schlossgarten 3, D-4400, Münster, Bundesrepublik Deutschland

    I. Tobüren-Bots & H. Hagedorn

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  1. I. Tobüren-Bots
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  2. H. Hagedorn
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Tobüren-Bots, I., Hagedorn, H. Untersuchungen über das Transportsystem für Thiamin bei Bacillus cereus . Arch. Microbiol. 113, 23–31 (1977). https://doi.org/10.1007/BF00428575

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  • DOI: https://doi.org/10.1007/BF00428575

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