Abstract
The hypothesis of an alternative pathway of sulphur amino acid synthesis as the basis of the prototrophy of sulphite reductase negative (Sr-) strains of Saccharomyces cerevisiae has been rejected. Met- mutants obtained after phenylmercuric nitrate treatment of Sr- strains accumulate H2S as the consequence of a metabolic block which leads to methionine auxotrophy. This mutation has been shown to be independent of the Sr locus. We assume that the molecular basis of the prototrophy of Sr- strains resides in a leaky missense induced in the Sr gene.
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Abbreviations
- PMN:
-
phenylmercuric nitrate
- HSP:
-
high H2S production
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This investigation was supported by a research grant of C.N.R. (Consiglio Nazionale delle Ricerche), Roma.
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Zambonelli, C., Mutinelli, P. & Pacchetti, G. Biosynthesis of sulphur amino acids in Saccharomyces cerevisiae . Arch. Microbiol. 102, 247–251 (1975). https://doi.org/10.1007/BF00428375
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DOI: https://doi.org/10.1007/BF00428375