Abstract
The present paper describes intracellular changes in α-mannosidase specific activity during Ca++-induced sporangium formation in the water mold Achlya bisexualis. The enzyme, which is concentrated in a cellular fraction with lysosome-like characteristics, undergoes a four-fold increase during sporangium differentiation. Addition of cycloheximide (100 μg/ml) or actinomycin D (10 μg/ml) at any time during the developmental sequence prevents further increase in the enzyme activity. These data suggest that coincident RNA and protein synthesis are essential for the accumulation of enzyme activity. Mixing of cell extracts from different developmental stages provides evidence that activators or inhibitors of the enzyme activity are not responsible for the enzyme activity evident at the different stages.
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Horgen, P.A., O'Day, D.H. The developmental patterns of lysosomal enzyme activities during Ca++-induced sporangium formation in Achlya bisexualis . Arch. Microbiol. 102, 9–12 (1975). https://doi.org/10.1007/BF00428338
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DOI: https://doi.org/10.1007/BF00428338