Abstract
Bacteria isolated from lake sediment samples reduced sodium selenite to elemental selenium. Finestructural observations were made on a number of different bacterial species cultured in the presence of sodium selenite. Examination of Escherichia coli and a Pseudomonas species revealed electron-dense deposits of irregular shape, composed of smaller units, within the cytoplasm but not on the cell wall and cell membrane. Cells of Aeromonas and Flavobacterium species exhibited conspicuous intranuclear fibrillary aggregates and different electron-dense inclusions. It appeared that the membrane structures were somewhat more easily stained in some bacterial cells after growth on agar plates containing sodium selenite. The deposits and fibrillary accumulations were interpreted to contain selenium on the basis of energy dispersive X-ray analysis. Control preparations and cells grown in the presence of sodium selenate were void of any fine-structural abnormalities. Alterations in fine structure are discussed in relation to the metabolism of selenium by bacterial cells and possible sites of inhibition.
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Abbreviations
- TEM:
-
transmission electron microscopy
- EDX:
-
energy dispersive X-ray
Refences
Bonhorst, C. W.: Selenium poisoning. Anion antagonisms in yeast as indicators of the mechanism of selenium toxicity. J. Agr. Food Chem. 3, 700–703 (1955)
Challenger, F.: Biological methylation. Chem. Rev. 36, 315–361 (1945)
Copeland, R.: Selenium: The unknown pollutant. Limnos 3, 7–9 (1970)
Cowie, D. B., Cohen, G. N.: Biosynthesis by Escherichia coli of active altered proteins containing selenium instead of sulfur. Biochim. biophys. Acta (Amst.) 26, 252–261 (1957)
Dempsey, E. W., Agate, F. J., Lee, M., Purkerson, M. L.: Analysis of submicroscopic structures by emitted X-rays. J. Histochem. Cytochem. 21, 580–586 (1973)
Falcone, G., Nickerson, W. J.: Reduction of selenite by intact yeast cells and cell free preparations. J. Bact. 85, 763–771 (1963)
Ganther, H. E., Corcoran, C.: Selenotrisulfides. III. Crosslinking of reduced pancreatic ribonuclease with selenium. Biochemistry 8, 2557 (1969)
Gerrard, T. L., Telford, J. N., Williams, H. H.: Detection of selenium deposits in Escherichia coli by electron microscopy. J. Bact. 119, 1057–1060 (1974)
Hoekstra, W. G.: Biochemical role of selenium. In: Trace element metabolism in animals, Vol. 2, W. G. Hoekstra, J. W. Suttie, H. E. Ganther, W. Mertz, eds., pp. 61–77. Baltimore: University Park Press 1974
Kumar, H. D., Prakash, G.: Toxicity of selenium to the blue-green algae, Anacystis nidulans and Anabaena variablis. Ann. Bot. 35, 697–705 (1971)
Levine, V. E.: The reducing properties of microorganisms with special reference to selenium compounds. J. Bact. 10, 217–263 (1925)
McCready, R. G. L., Campbell, J. N., Payne, J. I.: Selenite reduction by Salmonella heidelberg. Canad. J. Microbiol. 12, 703–714 (1966)
Nickerson, W. J.: Reduction of inorganic substances by yeasts. I. Extracellular reduction of sulfite by species of Candida. J. infect. Dis. 93, 43–56 (1953)
Painter, E. P.: The chemistry and toxicity of selenium compounds with special reference to the selenium problem. Chem. Res. 28, 179–213 (1941)
Rosenfield, I., Beath, O. A.: Selenium: New York: Academic Press 1964
Shrift, A.: Biochemical inter-relations between selenium and sulphur in plants and microorganisms. Fed. Proc. 20, 695–702 (1961)
Stahl, A. R.: Preliminary air pollution survey of selenium and its compounds. U. S. Dept. Health, Education and Welfare, NAPCA (1969)
Tuve, T., Williams, H. H.: Metabolism of selenium by Escherichia coli. J. biol. Chem. 236, 597–601 (1961)
Weissman, G. S., Trelease, S. F.: Influence of sulfur on the toxicity of selenium to Aspergillus. Amer. J. Bot. 42, 489–495 (1955)
Wong, P. T. S., Chau, Y. K., Luxon, P. L., Silverberg, B. A.: Methylation of heavy metals in the environment. Proceedings International Conference on Heavy Metals in the Environment, Toronto, C220-C221 (1975)
Zalokar, M.: Reduction of selenite by Neurospora. Arch. Biochem. Biophys. 44, 330–337 (1953)
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Silverberg, B.A., Wong, P.T.S. & Chau, Y.K. Localization of selenium in bacterial cells using TEM and energy dispersive X-ray analysis. Arch. Microbiol. 107, 1–6 (1976). https://doi.org/10.1007/BF00427860
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DOI: https://doi.org/10.1007/BF00427860