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Properties of the cell envelope and a cell-envelope protein of Pseudomonas facilis

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Abstract

The molecular weight of the protein moiety of a phospholipoprotein complex isolated from Pseudomonas facilis has been examined with a variety of sodium dodecylsulfate-polyacrylamide gel electrophoretic systems. A molecular weight of 35 000 was determined for the protein in all analyses. A 35 000-dalton protein was present in the EDTA extract of P. facilis and in the cytoplasmic and outer membrane fractions, but not in the lipopolysaccharide and peptidoglycan. Prior inoculation of mice with the phospholipoprotein complex led to a 7.5- to 15-fold increase in the LD50 when mice were subsequently inoculated with Salmonella typhimurium; this pathogen has a cell-surface protein which cross-reacts immunologically with antibody to the P. facilis phospholipoprotein complex.

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Abbreviations

KDO:

2-keto-3-deoxyoctanoate

LD50 :

the dosage of Salmonella typhimurium at which there is 50% survival in mice

LPS:

lipopolysaccharide

PLP:

phospholipoprotein

PPLP :

the protein moiety of PLP

SDS:

sodium dodecylsulfate

SDS-PAGE:

sodium dodecylsulfate-polyacrylamide gel electrophoresis

TMS:

trimethylsilyl

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Authors and Affiliations

  1. Biochemistry/Biophysics Program in the Department of Chemistry, Washington State University, 99164, Pullman, WA, USA

    Russell C. Jones & Bruce A. McFadden

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  1. Russell C. Jones
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  2. Bruce A. McFadden
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Jones, R.C., McFadden, B.A. Properties of the cell envelope and a cell-envelope protein of Pseudomonas facilis . Arch. Microbiol. 115, 37–43 (1977). https://doi.org/10.1007/BF00427842

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  • DOI: https://doi.org/10.1007/BF00427842

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