Abstract
Photosynthetically active membranes have been prepared from the thermophilic cyanobacterium Mastigogladus laminosus by treatment with lysozyme. The membranes were active in electron transport through photosystem I and II as well as in photophosphorylation and proton uptake. Cells were grown at 40°, 45° and 55°C respectively. The temperature optimum of oxygen evolution of whole cells was about 10°C higher than the growth temperature. In isolated membranes the temperature optimum for cyclic photophosphorylation was identical to the growth temperature of the cells whereas the optimum for photosystem II electron transport never exceeded 40°C. Photophosphorylation was inhibited by N, N′-dicyclohexyl carbodiimide (DCCD), carbonyl-cyanide-m-chlorophenylhydrazone and NH4Cl, whereas proton uptake was enhanced by DCCD. Electron transport was slightly inhibited by these treatments. The membranes could be stored for several weeks at-20°C in 50% glycerol without any loss in the activities.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Abbreviations
- DPIP:
-
2,6-dichlorophenolindophenol
- CCCP:
-
Carbonyl-cyanide-m-chlorophenylhydrazone
- DCCD:
-
N,N′-dicyclohexyl carbodiimide
- PMS:
-
N-methylphenazonium methosulfate
- DCMU:
-
3-(3′-4′-dichlorophenyl)-1,1-dimethylurea
- TMP:
-
20 mM Tris-HCl buffer pH 7.8, 10 mM MgCl2, 5 mM phosphate buffer pH 7.8
References
Avron, M.: Photophosphorylation as a tool for the synthesis of specifically labeled nucleotides. Anal. Biochem. 2, 535–543 (1961)
Binder, A., Locher, P., Zuber, H.: Concerning the large scale cultivation of the thermophilic cosmopolitan Mastigocladus laminosus in icelandic hot springs. Arch. Hydrobiol. 70, 541–555 (1972)
Binder, A., Tel-Or, E., Avron, M.: Photosynthetic activities of membrane preparations of the blue-green alga Phormidium luridum. Eur. J. biochem. 67, 187–196 (1976)
Binder, A., Bachofen, R.: Isolation and characterization of a coupling factor I ATPase of the thermophilic blue-green alga (cyanobacterium) Mastigocladus laminosus. FEBS Letters 104, 66–70 (1979)
Buchanan, B., Arnon, D.: Ferredoxin from photosynthetic bacteria, algae and higher plants. Methods Enzymol. 23, 413–440 (1971)
Castenholz, R. W.: The thermophilic Cyanophytes of Iceland and the upper temperature limlit. J. Phycol. 5, 360–368 (1969a)
Castenholz, R. W.: Thermophilic blue-green algae and the thermal environment. Bact. Rev. 33, 476–504 (1969b)
Fillingame, R.: Purification of the carbodiimide reactive protein component of the ATP energy transducing system of Escherichia coli. J. Biol. Chem. 251, 6630–6637 (1976)
Fork, D. C., Murata, N.: Studies on the effect of transition of the physical phase of membrane lipids on electron transport in the extreme thermophile Synechococcus lividus. Carnegie Institution Year Book 76, 222–226 (1977)
Mac Kinney, G.: Absorption of light by chlorophyll solutions. J. Biol. Chem. 140, 315–322 (1941)
McSwain, B., Tsujimoto, H. J., Arnon, D. L.: Effects of magnesium and chloride ions on light induced electron transport in membrane fragments from a blue green alga. Biochim. Biophys. Acta 423, 313–322 (1976)
Ono, T. A., Murata, N.: Temperature dependence of the photosynthetic activities in the thylakoid membranes from the blue green alga Anacystis nidulans. Biochim. Biophys. Acta 545, 69–76 (1979)
Oshima, T., Imahori, K.: Description of Thermus thermophilus, an non sporulating thermophilic bacterium from a japanese thermal spa. Int. J. of Systematic Bact. 24, 102–112 (1974)
Yamaoka, T., Satoh, K., Katoh, S.: Photosynthetic activities of a thermophilic blue-green alga. Plants and Cell Physiol. 19, 943–454 (1978)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Bohler, MC., Binder, A. Photosynthetic activities of a membrane preparation of the thermophilic cyanobacterium Mastigocladus laminosus . Arch. Microbiol. 124, 155–160 (1980). https://doi.org/10.1007/BF00427721
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00427721