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Side gland of Suncus murinus as a new model of sebaceous gland: 5α-reductase, androgen receptor, and nuclear androgen content in male and female animals

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Summary

The side gland of Suncus murinus is composed of well-developed sebaceous glands in both males and females. We measured the 5α-reductase activity, androgen receptor content, and the intranuclear concentrations of testosterone and dihydrotestosterone in this side gland taking it as a new experimental model of human sebaceous glands. Lineweaver-Burk plot analysis suggested the presence of two classes of 5α-reductase with different Km for testosterone in the homogenate. The enzyme activity was slightly higher in males than in females in the presence of a high concentration of testosterone. The levels of androgen receptors were approximately 40 and 30 fmol/mg protein in the cytosol and nuclei, respectively. The values did not differ significantly between males and females. The intranuclear concentration of dihydrotestosterone in the side gland was higher than that of testosterone in each sex. The intranuclear level of each of these two androgens in the female side gland was comparable to that in the male side gland despite the fact that the serum level of testosterone was much lower in the female. These data clearly indicate that the side gland is a typical target tissue for androgens in the female as well as in the male. Androgens other than testosterone may serve as precursors of dihydrotestosterone in the female side gland.

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Authors and Affiliations

  1. Department of Dermatology, Medical College of Oita, Oita-Prefecture, Japan

    S. Komada, S. Itami, S. Kurata & S. Takayasu

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  1. S. Komada
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  2. S. Itami
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  3. S. Kurata
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  4. S. Takayasu
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This work was supported by grant no. 61770752 from the Ministry of Education of Japan

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Komada, S., Itami, S., Kurata, S. et al. Side gland of Suncus murinus as a new model of sebaceous gland: 5α-reductase, androgen receptor, and nuclear androgen content in male and female animals. Arch Dermatol Res 280, 487–493 (1989). https://doi.org/10.1007/BF00427662

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  • DOI: https://doi.org/10.1007/BF00427662

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