Abstract
Factor F430 is a yellow compound of unknown structure present in methanogenic bacteria. It has recently been shown to contain nickel. In this communication the influence of the nickel concentration in the growth medium on the factor F430 content of Methanobacterium thermoautotrophicum and on the nickel content of factor F430 was studied. It was found: (1) The content of factor F430 in the cells was strongly dependent on the nickel concentration of the growth medium. Cells grown on media with 2.5 μM NiCl2 contained 28 times as much factor F430 per g as those grown on media with 0.075 μM NiCl2; (2) factor F430 was synthesized in nickel deprived cells only upon the addition of nickel Nickel uptake paralleled factor F430 synthesis; (3) independent of the nickel concentration in the growth medium, the extinction coefficient at 430 nm of factor F430 per mol nickel was always near 22,500 cm-1 (mol Ni)-1. These findings indicate that nickel is an essential component of factor F430.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Baalen, C. van, O'Donnell, R.: Isolation of a nickel-dependent blue-green alga. J. Gen. Microbiol. 105, 351–353 (1978)
Balch, W. E., Fox, G. E., Magrum, L. J., Woese, C. R., Wolfe, R. S.: Methanogens: Reevaluation of a unique biological group. Microbiol. Rev. 43, 260–296 (1979)
Bartha, R., Ordal, E. J.: Nickel-dependent chemolithotrophic growth of two Hydrogenomonas strains J. Bacteriol. 89, 1015–1019 (1965)
Bottari, E.: Complex formation between nickel(II) and alpha aminoacids. Ann. Chim. 66, 139–154 (1976)
Diekert, G. B., Graf, E. G., Thauer, R. K.: Nickel requirement for carbon monoxide dehydrogenase formation in Clostridium pasteurianum. Arch. Microbiol. 122, 117–120 (1979)
Diekert, G., Klee, B., Thauer, R. K.: Nickel, a component of factor F430 from Methanobacterium thermoautotrophicum. Arch. Microbiol. 124, 103–106 (1980)
Diekert, G., Thauer, R. K.: The effect of nickel on carbon monoxide dehydrogenase formation in Clostridium thermoaceticum and Clostridium formicoaceticum. FEMS Letters 7, 187–189 (1980)
Dixon, N. E., Gazzola, C., Blakely, R. L., Zerner, B.: Jack bean urease (EC 3.5.1.5). A metallo-enzyme. A simple biological role for nickel? J. Am. Chem. Soc. 97, 4131–4133 (1975)
Dixon, N. E., Gazzola, C., Blakely, R. L., Zerner, B.: Metal ions in enzymes using ammonia or amides. Science 191, 1144–1150 (1976)
Drake, H. L., Hu, S. I., Wood, H. G.: Purification of carbon monoxide dehydrogenase, a nickel enzyme from Clostridium thermoaceticum. J. Biol. Chem. (submitted)
Fishbein, W. N., Smith, M. J., Nagarajan, K., Scurzi, W.: The 1st natural nickel metallo enzyme urease. Fed. Proc. 35, 1643 (1976)
Fuchs, G., Stupperich, E., Thauer, R. K.: Acetate assimilation and the synthesis of alanine, aspartate and glutamate in Methanobacterium thermoautotrophicum. Arch. Microbiol. 117, 61–66 (1978)
Gunsalus, R. P., Wolfe, R. S.: Chromophoric factors F342 and F430 of Methanobacterium thermoautotrophicum. FEMS Letters 3, 191–193 (1978)
Jolly, P. W., Wilke, G.: The organic chemistry of nickel. Vol. 1: Organonickel complexes. New York, London: Academic Press Inc. 1974
Kirchgessner, M., Schnegg, A.: Zur Aktivität der Proteasen, Leucinarylamidase and α-Amylase im Pankreasgewebe bei Nickelmangel. Nutr. Metab. 23, 62–64 (1979)
Ose, D. E., Fridovich, E.: Superoxid dismutase: Reversible removement of manganese and its substitution by cobalt, nickel, or zinc. J. Biol. Chem. 251, 1217–1218 (1976)
Polacco, J. C.: Nitrogen metabolism in soybean tissue culture II. Urea utilization and urease synthesis require Ni2+. Plant Physiol. 59, 827–830 (1977)
Ray, W. J., Goodin, D. S., Ng, L.: Cobalt(II) and nickel(II) complexes of phosphoglucomutase. Biochemistry 11, 2800–2804 (1972)
Repaske, R., Repaske, A. C.: Quantitative requirements for exponential growth of Alcaligenes eutrophus. Appl. Environ. Microbiol 32, 585–591 (1976)
Schnegg, A., Kirchgessner, M.: Zur Essentialität von Nickel für das tierische Wachstum. Z für Tierphysiol., Tierernähr. Futtermittelk. 36, 63–74 (1975)
Schönheit, P., Moll, J., Thauer, R. K.: Nickel, cobalt, and molybdenum requirement for growth of Methanobacterium thermoautotrophicum. Arch. Microbiol. 123, 105–107 (1979)
Schönheit, P., Moll, J., Thauer, R. K.: Growth parameters (Ks, μmax, Ys) of Methanobacterium thermoautotrophicum. Arch. Microbiol. 127, 59–65 (1980)
Spears, J. W., Hatfield, E. E.: Nickel for ruminants. I. Influence of dietary nickel on ruminal urease activity. J. Animal Science 47, 1345–1350 (1978)
Spears, J. W., Hatfield, E. E., Fahey, G. C.: Nickel depletion in the growing ovine. Nutr. Rep. Internat. 18, 621–629 (1978a)
Spears, J. W., Smith, C. J., Hatfield, E. E.: Rumen bacterial urease requirement for nickel. J. Dairy Sci. 60, 1073–1076 (1978b)
Tabillion, R., Weber, F., Kaltwasser, H.: Nickel requirement for chemolithotrophic growth in hydrogen-oxidizing bacteria. Arch. Microbiol. 124, 131–136 (1980)
Thauer, R. K., Fuchs, G.: Methanogene Bakterien; Ungewöhnliche Zellkomponenten und Stoffwechselwege in einer Bakterien-gruppe mit phylogenetischer Sonderstellung. Naturwissenschaften. 66, 89–94 (1979)
Whitman, W. B., Wolfe, R. S.: Presence of nickel in factor F430 from Methanobacterium bryantii. Biochem. Biophys. Res. Commun. 92, 1196–1201 (1980)
Author information
Authors and Affiliations
Additional information
Dedicated to Professor Otto Kandler on the occasion of his 60th birthday
Rights and permissions
About this article
Cite this article
Diekert, G., Weber, B. & Thauer, R.K. Nickel dependence of factor F430 content in Methanobacterium thermoautotrophicum . Arch. Microbiol. 127, 273–277 (1980). https://doi.org/10.1007/BF00427204
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00427204