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Archives of Microbiology

, Volume 127, Issue 3, pp 245–252 | Cite as

NADH as electron donor for the photosynthetic membrane of Chlamydomonas reinhardii

  • Doris Godde
  • Achim Trebst
Article

Abstract

A chloroplast fraction from Chlamydomonas reinhardii cells can oxidize NADH in the light, unlike chloroplasts of higher plants. The Chlamydomonas preparation catalyzes electron flow from NADH to methylviologen or ferredoxin to evolve hydrogen (in the presence of a hydrogenase) or take up oxygen. The NADH photooxidation is sensitive to rotenone, dibromothymoquinone and dicyclohexylcarbodiimide. This suggests that a rotenone sensitive NADH dehydrogenase is coupled on the plastoquinone reduction site of the potosynthetic electron flow system. On sonication of the particles NADH photooxidation is lost but may be restored by a protein fraction from an acetone extract plus plastocyanin.

Key words

Chlamydomonas reinhardii Algae chloroplasts Photosynthetic electron transport Hydrogen metabolism NADH photooxidation 

Abbreviations

DAD

diaminodurene

DCCD

dicyclohexylcarbodiimide

DCMU

(3,3-dichlorphenyl)-N′·N′ dimethyl urea

DBMIB

dibromothymoquinone

DNP-INT

dinitro-phenylether of 2-iodo-4-nitrothymol

MV

methylviologen

chl

chlorophyll

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Copyright information

© Springer-Verlag 1980

Authors and Affiliations

  • Doris Godde
    • 1
  • Achim Trebst
    • 1
  1. 1.Lehrsuhl für Biochemie der PflanzenRuhr-Universität BochumBochumFederal Republic of Germany

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