Summary
TYMV-dsRNA has been studied by ORD and CD. The spectra indicate a highly ordered secondary structure which disappears upon heating or titrating to acid pH. No distinct acid structure as found for DNA could be observed.
Melting profiles of TYMV-dsRNA show a strong dependence on ionic strength which is analogous to synthetic polynucleotide complexes. Upon storage for several months the melting points drop considerably, without change in the ORD spectra. The two sets of melting points obtained for TYMV-dsRNA compare well with the results obtained in the literature: two series of melting points have been found for ds-RNA of a wide variety of origins. If plotted as a function of their G-C content, these data fall on two different lines with different slopes.
It is suggested that ds-RNA might exist in two different helical forms and that TYMV-dsRNA might undergo a conformational change in solution upon storage.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Arnott, S., M. H. F. Wilkins, W. Fuller, and R. Langridge: Molecular and crystal structures of double-helical RNA. II. Determination and comparison of diffracted intensities for the α and β crystalline forms of reovirus RNA and their interpretation in terms of groups of three RNA molecules. J. molec. Biol. 27, 525 (1967).
————: Molecular and crystal structures of double-helical RNA. III. An 11-fold molecular model and comparison of the agreement between the observed and calculated three-dimensional diffraction data for 10- and 11-fold models. J. molec. Biol. 27, 535 (1967).
———: Molecular and crystal structures of double-helical RNA. IV. Molecular packing in crystalline fibres. J. molec. Biol. 27, 549 (1967).
Billeter, M. A., C. Weissmann, and R. C. Warner: Replication of viral RNA. IX. Properties of double-stranded RNA from E. coli infected with bacteriophage MS-2. J. molec. Biol. 17, 145 (1966).
Bockstahler, L. E.: Biophysical studies on double-stranded RNA from TYMV-infected plants. Molec. gen. Genetics 100, 337 (1967).
Bové, J. M.: Virus de la Mosaique jaune du Navet: Synthèse asymétrique in vitro d'un segment de RNA viral. Thèse de Doctorat, Université de Paris. No d'enregistrement C.N.R.S. AO 1289 (1967).
—: TYMV-RNA Synthesis in vitro: Evidence for native ds-RNA. Biochem. biophys. Res. Commun. 32, 480 (1968).
Brahms, J., and W. F. H. M. Mommaerts: A study of conformation of nucleic acids in solution by means of circular dichroism. J. molec. Biol. 10, 73 (1964).
Burdon, R. H., M. A. Billeter, C. Weissmann, R. C. Warner, S. Ochoa, and C. A. Knight: Replication of viral RNA. V. Presence of a virusspecific ds-RNA in leaves infected with TMV. Proc. nat. Acad. Sci. (Wash.) 52, 768 (1964).
Cantor, C. R., S. R. Jaskunas, and I. Tinoco: Optical properties of RNA predicted from oligomers. J. molec. Biol. 20, 39 (1966).
Chamberlin, M. J., and D. L. Patterson: Physical and chemical characterization of the ordered complexes formed between poly (I), poly (C) and their deoxyribo-analogues. J. molec. Biol. 12, 410 (1965).
Courtois, Y., P. Fromageot, and W. Guschlbauer: Protonated polynucleotide structures. III. An ORD study of the protonation of DNA. Eur. J. Biochem. 6, 493 (1968).
Courtois, Y., D. Thiele, and W. Guschlbauer: Polynucleotides protonés. V. Etude sur la protonation des acides nucléiques et des polynucléotides par ORD et CD. Bull. Soc. Chim. biol. (Paris) sous presse (1968).
Erikson, R. L., E. Erikson, and J. A. Gordon: Structure and function of bacteriophage R 17 rI-RNA. I. Studies on sedimentation and infectivity. J. molec. Biol. 22, 257 (1966).
Fraenkel-Conrat, H., and C. Weissmann: In vitro synthesis of viral components. In: Molecular basis of virology (Fraenkel-Conrat, ed.), ACS Monograph No 164, p. 209. Reinhold 1968.
Franklin, R. M.: Purification and properties of the rI of the RNA bacteriophage R 17. Proc. nat. Acad. Sci. (Wash.) 55, 1504 (1966).
Gomatos, P. J., and I. Tamm: The secondary structure of reovirus RNA. Proc. nat. Acad. Sci. (Wash.) 49, 707 (1963).
——: Animal and plant viruses with ds-RNA. Proc. nat. Acad. Sci. (Wash.) 50, 878 (1963).
Granboulan, N., and R. M. Franklin: Electron microscopy of RNA rf and rI of the bacteriophage R 17. J. molec. Biol. 22, 173 (1966).
Guschlbauer, W., and Y. Courtois: pH induced changes in optical activity of guanine nucleosides. FEBS-Letters 1, 183 (1968).
Hofschneider, P. H., and P. Hausen: The replication cycle. In: Molecular basis of virology (Fraenkel-Conrat, ed.). ACS Monograph No 164, p. 169. Reinhold 1968.
Kaerner, H. C., u. H. Hoffmann-Berling: Die Bildung von ds-RNS zur Vermehrung eines RNS enthaltenden Bakteriophagen. Z. Naturforsch. 19b, 593 (1964).
Lamborg, M. R., P. C. Zamecnik, T. K. Li, J. Kägi, and B. L. Vallee: Anomalous ORD of sRNA and its relation to amino acid synthetase recognition. Biochemistry 4, 63 (1965).
Marmur, J., and P. Doty: Determination of the base composition of DNA from its thermal denaturation temperature. J. molec. Biol. 5, 109 (1962).
Massoulié, J.: Thermodynamique des associations de poly A et poly U en milieu neutre et alcalin. Eur. J. Biochem. 3, 428 (1968).
McPhie, P., and W. B. Gratzer: The ORD of ribosomes and their constituents. Biochemistry 5, 1310 (1966).
Michelson, A. M., J. Massoulié, and W. Guschlbauer: Synthetic polynucleotides. Prog. in Nucleic Acid Res. 6, 83 (1967).
Oriel, P. J., and J. Wilder: ORD of ds-RNA. Nature (Lond.) 214, 702 (1967).
Samejima, T., H. Hashizume, K. Imahori, I. Fujii, and K. I. Miura: ORD and CD of rice dwarf virus RNA. J. molec. Biol. 34, 39 (1968).
—: ORD and Conformation of DNA and RNA from various sources. J. biol. Chem. 240, 2094 (1965).
Sarkar, P. K., B. Wells, and J. T. Yang: A new CD band in nucleic acids and ribosomes. J. molec. Biol. 25, 563 (1967).
—: Optical activity and the conformation of poly I and several other polynucleotide complexes. Biochemistry 4, 1238 (1965).
Sato, T., Y. Kyogoku, S. Higuchi, Y. Mitsui, Y. Iitaka, M. Tsuboi, and K. I. Miura: A preliminary investigation on the molecular structure of RDV-RNA. J. molec. Biol. 16, 180 (1966)
Shipp, W., and R. Haselkorn: ds-RNA from tobacco leaves infected with TMV. Proc. nat. Acad. Sci. (Wash.) 52, 401 (1964).
Tomita, K. I., and A. Rich: X-ray diffraction investigations of complementary RNA. Nature (Lond.) 201, 1160 (1964).
Weissmann, C., and S. Ochoa: Replication of phage RNA. Progr Nucleic Acid Res. 6, 353 (1967).
Wilkins, M. H. F.: The molecular structure of DNA. In: “Deoxyribonucleic acid”-structure, synthesis and function. Proc. Renn. Ann. Soc. Chim. Phys. p. 15. Pergamon Press 1962.
Zimmer, Ch., G. Luck, H. Venner, and J. Fric: Studies on the conformation of protonated DNA. Biopolymers 6, 563 (1968).
Author information
Authors and Affiliations
Additional information
Communicated by G. Melchers
Boursier du Commissariat à l'Energie Atomique.
Rights and permissions
About this article
Cite this article
Guschlbauer, W., Courtois, Y., Bové, C. et al. Optical investigations on double stranded ribonucleic acid from turnip yellow mosaic virus. Molec. Gen. Genet. 103, 150–158 (1968). https://doi.org/10.1007/BF00427142
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00427142