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Genetic analysis of lon mutants of strain K-12 of Escherichia coli

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Summary

Following UV irradiation of AB1157 31 mucoid ultraviolet light UV sensitive mutants were isolated. These were all induced to form filaments by UV irradiation, i.e. they had all the phenotypic properties of Lon mutants. These lon mutants fell into two phenotypic classes based on their sensitivity to UV. The gene determining UV sensitivity and mucoidy in all mutants of both Class A and Class B was cotransducible with proC. Intra-class crosses by Pl transduction yielded no UV resistant recombinants. Inter-class crosses yielded UV resistant nonmucoid recombinants, the frequency depending on the direction of the cross. The data imply two adjacent blocks in the lon region of E. coli and the order of markers in this region is probably proC tsx lon Class A lon purE Class B.

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Authors and Affiliations

  1. Palo Alto Medical Research Foundation, Palo Alto, California

    John Donch & Joseph Greenberg

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  1. John Donch
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  2. Joseph Greenberg
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Communicated by G. S. Stent

This work was carried out under Public Health Service Grant CA 05687-08 from the National Cancer Institute.

Recipient of a Public Health Service Career Development Award.

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Donch, J., Greenberg, J. Genetic analysis of lon mutants of strain K-12 of Escherichia coli . Molec. Gen. Genet. 103, 105–115 (1968). https://doi.org/10.1007/BF00427138

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  • DOI: https://doi.org/10.1007/BF00427138

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