Abstract
The relationship between in vivo light absorption efficiency of whole cells and in vitro absorption efficiency of algal pigments has been examined experimentally in the marine diatom Thalassiosira sp. In vitro absorption spectra were obtained for cells disrupted by either ultrasonic treatment or high-pressure shearing stress in a low-temperature (-40°C) pressure cell. A dimensionless measure of the magnitude of the package effect (Q a *), calculated from the ratio of whole-cell to disrupted-cell absorption, ranged from about 0.5 at the blue absorption peak of chlorophyll a (λ=435 nm) to 0.7 at the red chlorophyll a peak (λ=670 nm) to 1.0 at the absorption minimum (λ=600 nm). Cell diameter was found to be an inappropriate measure of size for assessing the magnitude of the package effect. Instead, the effective optical diameter for calculation of intracellular self-shading was found to be less than the cell diameter. This observation is consistent with the fact that most algal pigments are contained within chloroplasts, and that chloroplast volume is necessarily smaller than cell volume.
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Literature cited
Aas, E.: The refractive index of phytoplankton, 61 pp. Oslo: University of Oslo, Institute of Geophysics 1981. (Internal publication, Ref. No. 46)
Atkinson, A. W., Jr. P. C. L. John and B. E. S. Gunning: The growth and division of the single mitochondrion and other organelles during the cell cycle of Chlorella, studied by quantitative stereology and three dimensional reconstruction. Protoplasma 81, 77–109 (1974)
Blasco, D., T. T. Packard and P. C. Garfield: Size dependence of growth rate, respiratory electron transport system activity, and chemical composition in marine diatoms in the laboratory. J. Phycol. 18, 58–63 (1982)
Bricaud, A. and A. Morel: Light attenuation and scattering by phytoplanktonic cells: a theoretical modeling. Appl. Optics 25, 571–580 (1986)
Bricaud, A., A. Morel and L. Prieur: Optical efficiency factors of some phytoplankters. Limnol. Oceanogr. 28, 816–832 (1983)
Bryant, F. D., B. A. Seiber and P. Latimer: Absolute absorption cross sections of cells and chloroplasts. Archs Biochem. Biophys. 135, 79–108 (1969)
Carder, K. L., G. Steward, J. H. Paul and G. A. Vargo: Relationships between chlorophyll and ocean color constituents as they affect remote-sensing reflectance models. Limnol. Oceanogr. 31, 403–413 (1986)
Carder, K. L., R. D., Tomlinson and G. F. Beardsley, Jr.: A technique for the estimation of indices of refraction of marine phytoplankters. Limnol. Oceanogr. 17, 833–839 (1972)
Das, M., E. Rabinowitch, L. Szalay and G. Papageorgiou: The “sieve effect” in Chlorella suspensions. J. phys. Chem., Wash. 71, 3543–3549 (1967)
Davies-Colley, R. J., R. D. Pridmore and J. E. Hewitt: Optical properties of some freshwater phytoplanktonic algae. Hydrobiologia 133, 165–178 (1986)
Fahnensteil, G. L., L. Sicko-Goad, D. Scavia and E. F. Stoermer: Importance of picoplankton in Lake Superior. Can. J. Fish. aquat. Sciences 43, 235–240 (1986)
Geider, R. J., T. Platt and J. A. Raven: Size dependence of growth and photosynthesis in diatoms: a synthesis. Mar. Ecol. Prog. Ser. 30, 93–104 (1986)
Graham, D. and R. M. Smillie: Chloroplasts (and lamellae): algal preparations. In: Methods in enzymology, pp 228–242. Ed. by A. San Pietro. New York: Academic Press 1971
Guillard, R. R. L. and J. H. Ryther: Studies on marine planktonic diatoms. I. Cyclotella nana Hustedt and Detonula confervaceae (Cleve) Gran. Can. J. Microbiol. 8, 229–239 (1962)
Heil, W. G. and H. Senger: Thylakoid-protein phosphorylation during the life cycle of Scenedesmus obliquus. Planta 167, 233–239 (1986)
Hitchcock, G. L.: A comparative study of size-dependent organic composition of marine diatoms and dinoflagellates. J. Plankton Res. 4, 363–377 (1982)
Hughes, D. E., J. W. T. Wimpenny and D., Lloyd: The disintegration of microorganisms. In: Methods in microbiology, pp 1–54. Ed. by J. R. Norris and D. W. Ribbons. London: Academic Press 1971
Hulst, van de, H. C.: Light scattering by small particles, 446 pp. New York: Wiley 1957
Jeffrey, S. W. and G. F. Humphrey: New spectrophotometric equations for determining chlorophylls, a, b, c 1 and c 2 in higher plants, algae and natural phytoplankton. Biochem. Physiol. Pfl. 167, 191–194 (1975)
Jeffrey, S. W. and M. Vesk: Effect of blue-green light on photosynthetic pigments and chloroplast structure in the marine diatom Stephanopyxis turris. J. Phycol. 13, 271–279 (1977)
Jeffrey, S. W. and M. Vesk: Chloroplast structural changes induced by white light in the marine diatom Stephanopyxis turris. J. Phycol. 14, 238–240 (1978)
Jennings, R. C. and G. Zucchelli: The influence of membrane stacking on light absorption by chloroplast. Photobiochem. Photobiophys. 9, 215–221 (1985)
Kiefer, D. A. and B. G. Mitchell: A simple, steady state description of phytoplankton growth based on absorption cross-section and quantum efficiency. Limnol. Oceanogr. 28, 770–776 (1983)
Kirk, J. T. O.: A theoretical analysis of the contribution of algal cells to the attenuation of light within natural waters. I. General treatment of pigmented cells. New Phytol. 75, 11–20 (1975a)
Kirk, J. T. O.: A theoretical analysis of the contribution of algal cells to the attenuation of light within natural waters. II. Spherical cells. New Phytol. 75, 21–36 (1975b)
Kirk, J. T. O.: A theoretical analysis of the contribution of algal cells to the attenuation of light within natural waters. III. Cylindrical and spheroidal cells. New Phytol. 77, 341–358 (1976)
Kirk, J. T. O.: Light and photosynthesis in aquatic ecosystems, 401 pp. Cambridge, Cambridge University Press 1983
Kirst, G. O. and D. Kramer: Cytological evidence for cytoplasmic volume control in Platymonas subcordiformis after osmotic stress. Pl. Cell Envir. 4, 455–462 (1981)
Köst, H.-P., M. Senser and G. Wanner: Effect of nitrate and sulfate starvation on Porphyridium cruentum cells. Z. Pflphysiol. 113, 231–249 (1984)
Latimer, P.: The deconvolution of absorption spectra of green plant materials — improved corrections for the sieve effect. Photochem. Photobiol. 38, 731–734 (1983)
Latimer, P.: A wave-optics effect which enhances light absorption by chlorophyll in vivo. Photochem. Photobiol. 40, 193–199 (1984)
Li, W. K. W., D. V. Subba-Rao, W. G. Harrison, J. C. Smith, J. J. Cullen, B. Irwin and T. Platt: Autotrophic picoplankton in the tropical ocean. Science, N.Y. 219, 292–295 (1983)
Mann, J. E. and J. Myers. On pigments growth and photosynthesis of Phaeodactylum tricornutum. J. Phycol. 4, 349–355 (1968)
Morel, A. and A. Bricaud: Theoretical results concerning light absorption in a discrete medium, and application to specific absorption of phytoplankton. Deep-Sea Res. 28A, 1375–1393 (1981)
Olson, R. A., W. H. Jennings and W. L. Butler: Molecular orientation: spectral dependence of dichroism of chloroplasts in vivo. Biochim. biophys. Acta 88, 318–330 (1964)
Osborne, B. A. and J. A. Raven: Light absorption by plants and its implications for photosynthesis. Biol. Rev. 61, 1–61 (1986a)
Osborne, B. A. and J. A. Raven: Growth light level and photon absorption by cells of Chlamydomonas rheinhardii, Dunaliella tertiolecta (Chlorophyceae, Volvocales), Scenedesmus obliquus (Chlorophyceae, Chlorococcales) and Euglena gracilis (Euglenophyceae, Euglenales). Br. Phycol. J. 21, 303–313 (1986b)
Owens, T. G. and P. G. Falkowski: Enzymatic degradation of chlorophyll a by marine phytoplankton in vitro. Phytochem. 21, 979–984 (1982)
Platt, T., D. V. Subba-Rao and B. Irwin: Photosynthesis of picoplankton in the oligotrophic ocean. Nature, Lond. 301, 702–704 (1983)
Rabideau, G. S., C. S. French and A. S. Holt: The absorption and reflection spectra of leaves, chloroplast suspensions, and chloroplast fragments as measured in an Ulbricht sphere. Am. J. Bot. 33, 769–777 (1946)
Rabinowitch, E.: Photosynthesis and related processes, Vol. II.2. 879 pp. New York: Interscience Publishers, Inc. 1956
Raven, J. A.: A cost-benefit analysis of photon absorption by photosynthetic unicells. New Phytol. 98, 593–625 (1984)
Rosen, B. H. and R. L. Lowe: Physiological and ultrastructural responses of Cyclotella meneghiniana (Bacillariophyta) to light intensity and nutrient limitation. J. Phycol. 20, 173–183 (1984)
Sicko-Goad, L.: A morphometric analysis of algal response to low dose, short-term heavy metal exposure. Protoplasma 116, 75–86 (1982)
Sicko-Goad, L. and E. F. Stoermer: A morphometric study of lead and copper effects on Diatoma tenue var. elongatum (Bacillariophyta). J. Phycol. 15, 316–321 (1979)
Smayda, T. J.: A quantitative analysis of the phytoplankton of the Gulf of Panama. II. On the relationship between 14C assimilation and the diatom standing crop. Bull. inter-Am. trop. Tuna Commn 9, 191–253 (1965)
Smayda, T. J.: From phytoplankton to biomass. In: Phytoplankton manual, pp 273–279. Ed. by A. Sournia. Paris: UNESCO 1978
Sournia, A.: Form and function in marine phytoplankton. Biol. Rev. 57, 347–394 (1982)
Strathmann, R. R.: Estimating the organic carbon content of phytoplankton from cell volume or plasma volume. Limnol. Oceanogr. 12, 411–418 (1967)
Taguchi, S.: Relationship between photosynthesis and cell size of marine diatoms. J. Phycol. 12, 185–189 (1976)
Takahashi, M. and P. K. Bienfang: Size structure of phytoplankton biomass and photosynthesis in subtropical Hawaiian waters. Mar. Biol. 76, 203–211 (1983)
Thinh, L.-V.: Effect of irradiance on the physiology and ultrastructure of the marine cryptomonad, Cryptomonas strain Lis (Cryptophyceae). Phycologia 22, 7–11 (1983)
Thorne, S. W., J. T. Duniec and J. A. Lee: Absorbance and fluorescence changes in relation to light scattering changes in chloroplast thylakoid membranes. Photobiochem. Photobiophys. 5, 71–78 (1983)
Vesk, M. and S. W. Jeffrey: Effect of blue-green light on photosynthetic pigments and chloroplast structure in unicellular marine algae from six classes. J. Phycol. 13, 280–288 (1977)
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Communicated by J. Mauchline, Oban
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Geider, R.J., Osborne, B.A. Light absorption by a marine diatom: experimental observations and theoretical calculations of the package effect in a small Thalassiosira species. Mar. Biol. 96, 299–308 (1987). https://doi.org/10.1007/BF00427030
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DOI: https://doi.org/10.1007/BF00427030