, Volume 63, Issue 1, pp 29–32 | Cite as

Effects of chlordiazepoxide on maze performance of rats subjected to undernutrition in early life

  • J. M. Celedon
  • M. Colombo
Original Investigations


The purpose of this research was to study the isolated effects of and interactions between undernutrition and emotional reactivity upon maze performance in rats. This work compares the effects of chlordiazepoxide on learning performance in two groups of adults rats: one undernourished early in life and then rehabilitated, and the other normally nourished. Chlordiazepoxide was used at 5 mg/kg body weight administered i.p. 30 min before each test. The response was evaluated in a Hebb-Williams maze. Water was used as reinforcement. Chlordiazepoxide improved performance in the early undernourished group 197±55.9 errors to 149±33.6 (mean±SD). Chlordiazepoxide impaired performance of the normal group from 107±22.7 errors to 197±42.2 errors. Analysis of variance gave an F=6.64, which is significant (P<0.01). This supports the hypothesis that the high emotional level of previously undernourished subjects acts negatively upon problem-solving performance.

Key words

Learning performance Chlordiazepoxide Hebb-Williams maze Undernutrition Rats 


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  1. Baird, A., Widdowson, E. M., Cowley, J. J.: Effects of calorie and protein deficiencies early in life on the subsequent learning ability of rats. Br. J. Nutr. 25, 391–403 (1971)Google Scholar
  2. Barnes, R., Neely, C. D., Kwong, E., Lavanden, B., Frankova, S.: Post-natal nutrition deprivations and determinants of adult rat behaviour towards food, its consumption and utilization. J. Nutr. 96, 467–476 (1968)Google Scholar
  3. Broadhurst, P. L.: Emotionality and the Yerkes-Dodson law. J. Exp. Psychol. 54, 345–352 (1957)Google Scholar
  4. Brofenbrenner, U.: Early deprivation in mammals: a cross species analysis. In: Early experiences and behaviours, G.Newton (ed.). Springfield, Ill. Charles C. Thomas 1968Google Scholar
  5. Celedon, J. M., Santander, M., Colombo, M.: Long term effects of early undernutrition and environmental stimulation on learning ability of adults rats. J. Pediatr. 91, 671–672 (1977)Google Scholar
  6. Cowley, J. J., Griesel, R. D.: The effect of growth and behaviour of rehabilitating first and second generation low protein rats. Anim. Behav. 14, 506–517 (1966)Google Scholar
  7. Dantzer, R., Baldwin, B. A.: Effects of chlordiazepoxide on heart rate and behavioural suppression in pigs subjected to operant conditioning procedures. Psychopharmacologia 37, 160–177 (1974)Google Scholar
  8. Guthrie, H. A.: Severe undernutrition in early infancy and behaviour in rehabilitated albino rats. Physiol. Behav. 3, 610 (1968)Google Scholar
  9. Hebb, D. O., Williams, K.: A method of rating animal intelligence. J. Genet. Psychol. 34, 59–65 (1946)Google Scholar
  10. Leathwood, P., Bush, M. S., Mauron, J.: The effects of chlordiazepoxide on avoidance performance of mice subjected to undernutrition or handling stress in early life. Psychopharmacologia 41, 105–109 (1975)Google Scholar
  11. Levine, S., Wiener, S.: A critical analysis of data on malnutrition and behavioural deficits. Adv. Pediat. 22, 113–136 (1976)Google Scholar
  12. Levitsky, D. A., Barnes, R. H.: Effect of early malnutrition on the reaction of adult rats to aversive stimuli. Nature 225, 468–469 (1970)Google Scholar
  13. Levitsky, D. A., Barnes, R. H.: Malnutrition and animal behaviour. In: Nutrition, development and social behaviour, (DHEW) Publication NIH 73-242. Washington, DC: US Government Printing Office 1973Google Scholar
  14. Lynch, A.: Passive avoidance behaviour and response threshold in adult male rats after early post-natal undernutrition. Physiol. Behav. 16, 2–32 (1976)Google Scholar
  15. Miczek, K. A., Lau, P.: Effects of scopolamine, physostigmine and chlordiazepoxide on punished and extinguished water consumption in rats. Psychopharmacologia 42, 263–269 (1975)Google Scholar
  16. Ottinger, D. R., Tanabe, G.: Maternal food restriction. Effects on offspring behaviour and development. Dev. Psychobiol. 2, 7–9 (1969)Google Scholar
  17. Rabinovich, M. S., Rosvold, H. E.: A closed field intelligence test for rats. Can. J. Psychol. 5, 122–128 (1951)Google Scholar
  18. Randt, C. T., Derby, B. M.: Behavioural and brain correlation in early life nutritional deprivation. Arch. Neurol. 28, 167–172 (1973)Google Scholar
  19. Semiginovsky, B., Myslivecek, J., Springer, V., Rokyta, R.: Testing of emotionality in animals with different levels of nutrition. Act. Nerv. Super. 11, 282–284 (1969)Google Scholar
  20. Simonson, M., Stephan, J. P., Hanson, H. M., Chow, B.: Open fields studies in offspring of underfeed mother rats. J. Nutr. 101, 331–336 (1971)Google Scholar
  21. Slob, A. K., Snow, C. E., Natris-Mathot, E.: Absence of behavioural deficits following neonatal undernutrition in the rat. Dev. Psychobiol. 6, 177–186 (1973)Google Scholar
  22. Smart, J. L.: Activity and exploratory behaviour of adult offspring of undernourished mother rats. Dev. Psychobiol. 7, 315–321 (1974)Google Scholar
  23. Smart, J. L.: Early life malnutrition and later learning ability. A critical analysis. In: Genetics, environment and intelligence, A. Oliverio (ed.), pp. 215–235. Amsterdam: North-Holland Biomedical Press 1977Google Scholar
  24. Smart, J., Dobbing, J.: Increased thirst and hunger in adult rats undernourished as infants: An alternative explanation. Br. J. Nutr. 37, 421–430 (1977)Google Scholar
  25. Smart, J. L., Dobbing, J., Adlard, B. P., Lynch, A., Sands, J.: Vulnerability of developing brain: Relative effects of growth restriction during the fetal and suckling periods on behaviour and brain composition of adult rats. J. Nutr. 103, 1327–1338 (1973)Google Scholar
  26. Smart, J. L., Whatson, T. S., Dobbing, J.: Thresholds of response to electric shock in previously undernourished rats. Br. J. Nutr. 34, 511–516 (1975)Google Scholar
  27. Soubrie, L., de Angelis, L., Simon, P., Boissier, J. R.: Effects des anxioly tigues sur la prise de boisson en situation nouvelle et familiere. Psychopharmacology 50, 41–45 (1976)Google Scholar
  28. Yamaguchi, K., Iwahara, S.: Effects of chlordiazepoxide upon differential heart rate conditioning in rats. Psychopharmacologia 39, 71–79 (1974)Google Scholar
  29. Yerkes, R. M., Dodson, J. D.: The relations of strength of stimulus to rapidity of habit-formation. J. Comp. Neurol. Psychol. 18, 459–482 (1908)Google Scholar
  30. Zimmermann, R. R., Wells, A. M.: Performance of malnourished rats on the Hebb-Williams closed-field maze learning task. Percept. Mot. Skills. 33, 1043–1050 (1971)Google Scholar

Copyright information

© Springer-Verlag 1979

Authors and Affiliations

  • J. M. Celedon
    • 1
  • M. Colombo
    • 1
  1. 1.Instituto de Nutricion y Tecnologia de los AlimentosUniversidad de ChileSantiago 11Chile

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