Psychopharmacologia

, Volume 37, Issue 1, pp 67–79 | Cite as

Opposed behavioral syndromes in rats with partial and more complete central serotonergic lesions made with 5,6 dihydroxytryptamine

  • Jaime Diaz
  • Gaylord Ellison
  • David Masuoka
Animal Studies

Abstract

Rats with central 5HT lesions made with multiple small injections of 5,6 dihydroxytryptamine (6×10 Μg 5,6 DHT) rear more but locomote less than Controls in novel environments, are more reactive to visual stimulation, and consume small, frequent meals. A converse behavioral syndrome is observed in rats in which smaller lesions are made (3×10 Μg 5,6 DHT) and a longer recovery period allowed. These latter animals show increased locomotion and decreased rearing in novel environments, consume large, infrequent meals, and demonstrate an exaggerated responsivity to the analgesic properties of morphine. It is proposed that the behavioral syndrome of rats with partially damaged, supersensitive circuity may clarify some paradoxical aspects of chronic 5HT disruptions.

Key words

5HT Lesions 5,6 Dihydroxytryptamine Exaggerated Recovery and Supersensitivity Animal Models Chronic Anxiety and Autism 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Baumgarten, H. G., Bjorklund, A., Lachenmayer, L., Nobin, A., Stenevi, U.: Long-lasting selective depletion of brain serotonin by 5,6-dihydroxytryptamine. Acta physiol. scand., Suppl. 373 (1971)Google Scholar
  2. Baumgarten, H. G., Lachenmayer, L., Bjorklund, A., Nobin, A., Rosengren, E.: Long-term recovery of serotonin concentration in the rat CNS following 5,6 dihydroxytryptamine. Life Sci. 12, 357–364 (1973)Google Scholar
  3. Baumgarten, H. G., Schlossberger, H. G.: Effects of 5,6 dihydroxytryptamine on brain monoamine neurons in the rat. In: Serotonin and behavior. J. Barchas and E. Usdin, Eds., pp. 209–224. New York: Academic Press 1973Google Scholar
  4. Bjorklund, A., Nobin, A., Stenevi, U.: Regeneration of central serotonin neurons after axonal degeneration induced by 5,6-dihydroxytryptamine. Brain Res. 50, 214–220 (1973)Google Scholar
  5. Brodie, B., Shore, P.: A concept for a role of serotonin and norepinephrine as chemical mediators in the brain. Ann. N.Y. Acad. Sci. 66, 631–642 (1957)Google Scholar
  6. Cannon, W. B., Rosenblueth, A.: The supersensitivity of denervated structures. New York: Macmillan 1949Google Scholar
  7. Cooper, B. R., Breese, G. R., Howard, J. L., Grant, L. D.: Enhanced behavioral depressant effects of reserpine and α-methyltyrosine after 6-hydroxydopamine treatment. Psychopharmacologia (Berl.) 27, 99–110 (1972)Google Scholar
  8. Dunnett, C. W.: New tables for multiple comparisons with a control. Biometrics 20, 482–491 (1964)Google Scholar
  9. Ellison, G. D., Bresler, D. E.: Tests of emotional behavior in rats following depletion of norepinephrine, of serotonin, or of both. Psychopharmacologia (Berl.) 34, 275–288 (1974)Google Scholar
  10. Fernstrom, J. D., Wurtman, R. J.: Brain serotonin content: increase following ingestion of carbohydrate diet. Science 174, 1023–1024 (1971)Google Scholar
  11. Fleming, R. M., Clark, W. G., Fenster, E. D., Towne, J. C.: Single extraction method for the simultaneous fluorimetric determination of serotonin, dopamine, and norepinephrine in brain. Analyt. Chem. 37, 692–696 (1965)Google Scholar
  12. Glowinski, J., Iversen, L. L.: Regional studies of catecholamines in the rat brain. I. The disposition of (3H) norepinephrine, (3H) dopamine, and (3H) dopa in various regions of rat brain. J. Neurochem. 13, 655–669 (1966)Google Scholar
  13. Hess, W. R.: Diencephalon. Autonomic and extrapyramidal functions. New York: Greene and Stratton 1954Google Scholar
  14. Houser, V. P., Pare, W. P.: A method for determining the aversive threshold in the rat using repeated measures: tests with morphine sulfate. Behav. Res. Meth. Instr. 4, 135–137 (1972)Google Scholar
  15. Jackson, H.: The Croonian Lectures on evolution and dissolution of the nervous system. Brit. med. J. 1, 591, 660, 703 (1884)Google Scholar
  16. Jouvet, M.: The role of monoamines and acetylcholine containing neurons in the regulation of the sleep waking cycle. Ergebn. Physiol. 64, 166–307 (1972)Google Scholar
  17. Katzman, R., Bjorklund, A., Owmon, C., Stenevi, U., West, K.: Evidence for regenerative axon sprouting of central catecholamines neurons in the rat mesencephalon following electrolytic lesions. Brain Res. 25, 579–597 (1971)Google Scholar
  18. Nakamura, K., Thoenen, H.: Increased irritability: a permanent behavior change induced in the rat by intraventricular administration of 6-hydroxydopamine. Psychopharmacologia (Berl.) 24, 359–372 (1972)Google Scholar
  19. Ritvo, E. R., Yuwiler, A., Geller, E., Ornitz, E. M., Saeger, K., Plotkin, S.: Increased blood serotonin and platelets in early infantile autism. Arch. gen. Psychiat. 23, 566–581 (1970)Google Scholar
  20. Simmonds, M., Uretsky, N.: Central effects of 6-hydroxydopamine on the body temperature of the rat. Brit. J. Pharmacol. 40, 630–638 (1970)Google Scholar
  21. Sorenson, C. A., Ellison, G.: Nonlinear changes in activity and emotional reactivity scores following central noradrenergic lesions in rats. Psychopharmacologia (Berl.) 23, 313–326 (1973)Google Scholar
  22. Tenen, S. S.: Antagonism of the analgesic effect of morphine and other drugs by PCPA, a serotonin depletor. Psychopharmacologia (Berl.) 12, 278–285 (1968)Google Scholar
  23. Tinbergen, E. A., Tinbergen, N.: Early childhood autism: an ethological approach. Berlin: Parey 1972Google Scholar
  24. Uretsky, J. J., Simmonds, M. A., Iverson, L. L.: Changes in the retention and metabolism of 3H-l-norepinephrine in rat brains in vivo after 6-hydroxydopamine pretreatments. J. Pharmacol. exp. Ther. 176, 489–496 (1971)Google Scholar
  25. Weissman, A.: Behavioral pharmacology of p-chlorophenylalanine (PCPA). In: J. Barchas and E. Usdin, Eds., Serotonin and behavior, pp. 235–248. New York: Academic Press 1973Google Scholar
  26. Winer, B. J.: Statistical principles in experimental design. New York: McGraw-Hill 1971Google Scholar
  27. Wyatt, R. J., Vaughan, T., Kaplan, J., Galanter, M., Green, R.: 5-Hydroxytryptophan and chronic schizophrenia—a preliminary study. In: Serotonin and behavior. J. Barchas and E. Usdin, Eds., pp. 487–498. New York: Academic Press 1973Google Scholar
  28. Zucker, M., Howard, W.: A transistorized body-capacitance relay for ecobehavioral studies. Anim. Behav. 16, 65–66 (1968)Google Scholar

Copyright information

© Springer-Verlag 1974

Authors and Affiliations

  • Jaime Diaz
    • 1
    • 2
  • Gaylord Ellison
    • 1
    • 2
  • David Masuoka
    • 1
    • 2
  1. 1.Department of PsychologyUniversity of CaliforniaLos Angeles
  2. 2.Neuropharmacology Research LaboratoryV. A. HospitalSepulveda

Personalised recommendations