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Archives of Dermatological Research

, Volume 281, Issue 2, pp 105–110 | Cite as

Biochemical and biological profile of a new enzyme preparation from antarctic krill (E. superba) suitable for debridement of ulcerative lesions

  • J. -E. Anheller
  • L. Hellgren
  • B. Karlstam
  • J. Vincent
Original Contributions

Summary

A protease extract from Antarctic krill (E. superba) intended as a new enzymatic debrider for necrotic ulcers has been characterized by sodium dodecyl sulphate polyacrylamide gradient gel electrophoresis and fast protein liquid chromatography. The predominant enzymes in the preparation represent trypsin-like activity associated with three serine proteinases. In addition two carboxypeptidases A and B are present as cooperative enzymes for a more complete breakdown of complex proteinaceous substrates. Biological studies on a well-defined substrate (fibrin) originating from leg ulcers, demonstrated more effective degradation by krill enzymes than bovine trypsin, a common component in marketed enzymatic debriders. These findings support previously in vitro/in vivo studies in an animal model (rat) using excised rat skin as “necrotic” tissue.

Key words

Antarctic krill (E. superbaProteases Biochemical/biological profile Enzymatic debridement 

Abbreviations

TAME

p-toluene-sulfonyl-l-arginine methyl ester; hipp-l-phe hippuryl-l-phenylalanine; hipp-l-arg, hippuryl-l-arginine

Cpase

carboxypeptidase

SDS

sodium dodecyl sulphate

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Copyright information

© Springer-Verlag 1989

Authors and Affiliations

  • J. -E. Anheller
    • 1
  • L. Hellgren
    • 2
  • B. Karlstam
    • 1
  • J. Vincent
    • 2
  1. 1.Pharmacia ABUppsalaSweden
  2. 2.Department of DermatologyUniversity of UmeåUmeåSweden

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