Archives of Dermatological Research

, Volume 281, Issue 2, pp 105–110 | Cite as

Biochemical and biological profile of a new enzyme preparation from antarctic krill (E. superba) suitable for debridement of ulcerative lesions

  • J. -E. Anheller
  • L. Hellgren
  • B. Karlstam
  • J. Vincent
Original Contributions


A protease extract from Antarctic krill (E. superba) intended as a new enzymatic debrider for necrotic ulcers has been characterized by sodium dodecyl sulphate polyacrylamide gradient gel electrophoresis and fast protein liquid chromatography. The predominant enzymes in the preparation represent trypsin-like activity associated with three serine proteinases. In addition two carboxypeptidases A and B are present as cooperative enzymes for a more complete breakdown of complex proteinaceous substrates. Biological studies on a well-defined substrate (fibrin) originating from leg ulcers, demonstrated more effective degradation by krill enzymes than bovine trypsin, a common component in marketed enzymatic debriders. These findings support previously in vitro/in vivo studies in an animal model (rat) using excised rat skin as “necrotic” tissue.

Key words

Antarctic krill (E. superbaProteases Biochemical/biological profile Enzymatic debridement 



p-toluene-sulfonyl-l-arginine methyl ester; hipp-l-phe hippuryl-l-phenylalanine; hipp-l-arg, hippuryl-l-arginine




sodium dodecyl sulphate


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Bucht A, Karlstam B (1986) Immunological characterization of three highly purified trypsin-like enzymes from Antarctic krill (Euphasia superba). Biol Chem Hoppe-Seyler [Suppl] 367:366Google Scholar
  2. 2.
    Bucht A, Johansson B, Karlstam B (1986) Separation and identification of proteases from Antarctic krill (Euphausia superba). 6th Int Symp HPLC Proteins, Peptides and Polynucleotides, Baden-Baden, pp 34Google Scholar
  3. 3.
    Campbell D, Hellgren L, Karlstam B, Vincent J (1987) Debriding ability of a novel multi-enzyme preparation isolated from Antarctic krill (Euphausia superba). Experientia 43:578–579Google Scholar
  4. 4.
    Falanga V, Eaglstein WH (1986) A therapeutic approach to venous ulcers. J Am Acad Dermatol 14:777–784Google Scholar
  5. 5.
    Folk JE, Schirmer EW (1963) The porcine pancreatic carboxypeptidase A system. J Biol Chem 238:3884–3894Google Scholar
  6. 6.
    Folk JE, Piez KA, Carroll WR, Gladner JA (1960) Carboxypeptidase B: purification and characterization of the porcine enzyme. J Biol Chem 235:2272–2277Google Scholar
  7. 7.
    Hellgren L, Mohr V, Vincent J (1986) Proteases of Antarctic krill — a new system for effective enzyme debridement of necrotic ulcerations. Experientia 42:403–404Google Scholar
  8. 8.
    Hellgren L, Mohr V, Vincent J (1987) Krill enzymes — a new debrider for necrotic ulcers. 17th World Congress of Dermatology, Berlin, pp 196Google Scholar
  9. 9.
    Johansson B, Karlstam B (1986) Purification and identification of carboxypeptidase A and B from Antarctic krill (Euphausia superba). Biol Chem Hoppe-Seyler [Suppl] 367:366Google Scholar
  10. 10.
    Kunitz MJ (1947) Crystalline soybean trypsin inhibitor. Gen Physiol 30:291–309Google Scholar
  11. 11.
    Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275Google Scholar
  12. 12.
    Osnes KK (1985) Peptide hydrolases of Antarctic krill (Euphausia superba). Thesis, Norwegian Institute of Technology, TrondheimGoogle Scholar
  13. 13.
    Rick W (1974) In: Bergmeyer HU (ed) Methods of enzymatic analysis, vol II, 2nd edn. Academic Press, New York, pp 1013–1023Google Scholar

Copyright information

© Springer-Verlag 1989

Authors and Affiliations

  • J. -E. Anheller
    • 1
  • L. Hellgren
    • 2
  • B. Karlstam
    • 1
  • J. Vincent
    • 2
  1. 1.Pharmacia ABUppsalaSweden
  2. 2.Department of DermatologyUniversity of UmeåUmeåSweden

Personalised recommendations