Chromosoma

, Volume 71, Issue 1, pp 65–80 | Cite as

Chromosome variation in the plethodontid salamander, Aneides ferreus

  • James Kezer
  • Stanley K. Sessions
Article

Abstract

Karyotype variation in the plethodontid salamander, Aneides ferreus, has been analysed. 358 individuals from 14 populations, representing the major portion of the range of this salamander, have been karyologically examined. In A. ferreus, n=14. When the chromosomes are arranged in a decreasing relative length series, the karyotype is heteromorphic with respect to chromosome number 13, which may be either telocentric (T) or subtelocentric (ST). Variation in the heteromorphism over the range of the species is sex related, and probably also reflects relative population sizes. The heteromorphism in the isolated populations of A. ferreus on Vancouver Island, British Columbia, Canada, resembles a WZfemale/ZZmale sex chromosome dimorphism, suggesting the possibility that chromosome number 13 may be involved in sex determination in this population. The possibility that chromosome number 13 is involved in sex determination in all populations of A. ferreus is discussed. Our data suggest that the ancestral A. ferreus karyotype was homomorphic for T (T/T), and that the ST was derived from the T by a pericentric inversion. In peripheral populations, only the “W homologue” has been affected, whereas in central populations both the “W” and the “Z” chromosomes have been rearranged. Comparisons are made with other species of Aneides for which karyological information is available, and it is concluded that chromosome rearrangements have played an important role in the evolution of the genus. In C-banded chromosomes of A. ferreus, staining is most intense at the centromere regions of the larger chromosomes and is absent only in some of the smaller chromosomes. Implications of this C-banding pattern are discussed.

Keywords

Chromosome Number Chromosome Rearrangement Centromere Region Central Population Relative Population 

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References

  1. Arrighi, F.E., Hsu, T.C.: Localization of heterochromatin in human chromosomes. Cytogenetics 10, 81–86 (1971)Google Scholar
  2. Callan, H.G.: Chromosomes and nucleoli of the axolotl, Ambystoma mexicanum. J. Cell Sci. 1, 85–108 (1966)Google Scholar
  3. Conger, H.G., Fairchild, L.M.: A quick-freeze method for making smear slides permanent. Stain Technol. 28, 281–283 (1953)Google Scholar
  4. Humphrey, R.R.: Sex determination in ambystomid salamanders: a study of the progeny of females experimentally converted into males. Amer. J. Anat. 76, 33–66 (1945)Google Scholar
  5. King, M., Rofe, R.: Karyotypic variation in the Australian gekko Phyllodactylus marmoratus (Gray) (Gehkonidae: Reptilia). Chromosoma (Berl.) 54, 75–87 (1976)Google Scholar
  6. Lacroix, J.-C.: Etude descriptive des chromosomes en écouvillon dans le genre Pleurodeles (Amphibien, urodèle). Ann. Embryol. Morphog. 1, 179–202 (1968 a)Google Scholar
  7. Lacroix, J.-C.: Variations expérimentales ou spontanées de la morphologie et de l'organisation des chromosomes en écouvillon dans le genre Pleurodeles (Amphibien, urodèle). Ann. Embryol. Morphog. 1, 205–248 (1968 b)Google Scholar
  8. Lacroix, J.-C.: Mise en évidence sur les chromosomes en écouvillon de Pleurodeles poireti Gervais, Amphibien urodèle, d'une structure liée au sexe, identifiant le bivalent sexual et marquant le chromosome W. C.R. Acad. Sci. (Paris) 271D, 102–104 (1970)Google Scholar
  9. León, P.E., Kezer, J.: Localization of 5S RNA genes on chromosomes of plethodontid salamanders. Chromosoma (Berl.) 65, 213–230 (1978)Google Scholar
  10. Levan, A., Fredga, K., Sandberg, A.A.: Nomenclature for centromeric position on chromosomes. Hereditas (Lund) 52, 201–220 (1964)Google Scholar
  11. Macgregor, H.C., Jones, C.: Chromosomes, DNA sequences, and evolution in salamanders of the genus Aneides. Chromosoma (Berl.) 63, 1–9 (1977)Google Scholar
  12. Mancino, G., Nardi, I.: Chromosomal heteromorphism and female heterogamety in the marbled newt Triturus marmoratus (Latreille, 1800). Experientia (Basel) 27, 821–822 (1971)Google Scholar
  13. Mayr, E.: Animal species and evolution. Boston: Belknap press of Harvard University 1963Google Scholar
  14. Morescalchi, A.: Conferma della presenza de eterocromosomi in Xenopus laevis Daudin. Rend. Acc. Sc. Fis. Mat. (Serie 4) 30, 310–314 (1963)Google Scholar
  15. Morescalchi, A., Olmo, E., Serra, V.: Chromosomes and DNA of the ambystomatoid salamanders. Experientia (Basel) 30, 619–620 (1974)Google Scholar
  16. Nardi, I., Ragghianti, M., Mancino, G.: Characterization of the lampbrush chromosomes of the marbled newt Triturus marmoratus (Latreille, 1800). Chromosoma (Berl.) 37, 1–22 (1972)Google Scholar
  17. Ohno, S.: Sex chromosomes and sex-linked genes. Berlin-Heidelberg-New York: Springer 1967Google Scholar
  18. Stebbins, R.C.: Amphibians of western North America. Berkely: University of California Press 1951Google Scholar
  19. Stephenson, E.M., Robinson, E.S., Stephenson, N.S.: Karyotype variation within the genus Leiopelma (Amphibia: Anura). Canad. J. Genet. Cytol. 14, 691–702 (1972)Google Scholar
  20. Thorgaard, G.G.: Heteromorphic sex chromosomes in male rainbow trout. Science 196, 900–902 (1977)Google Scholar
  21. Wake, D.B.: Comparative osteology of the plethodontid salamanders genus Aneides. J. Morph. 113, 77–118 (1963)Google Scholar
  22. Wake, D.B.: Comparative osteology and evolution of the lungless salamanders, family Plethodontidae. Mem. Southern Calif. Acad. Sci. 4, (1966)Google Scholar
  23. Weiler, C., Ohno, S.: Cytological confirmation of female heterogamety in the African water frog (Xenopus laevis). Cytogenetics 1, 217–223 (1962)Google Scholar
  24. White, M.J.D.: Animal cytology and evolution, 3rd edit. London: Cambridge University Press 1973Google Scholar

Copyright information

© Springer-Verlag 1979

Authors and Affiliations

  • James Kezer
    • 1
  • Stanley K. Sessions
    • 1
  1. 1.Department of BiologyUniversity of OregonEugeneUSA

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