Comparative Haematology International

, Volume 2, Issue 3, pp 162–165 | Cite as

The radioimmunoassay of histamine release from circulating basophils in an in vitro study as support for the in vivo active systemic anaphylaxis test in the guinea pig

  • D. Salauze
  • A. Hery
  • M. Corroller
Original Articles


An in vitro anaphylaxis test is described which explores the ability of compound re-challenge to induce histamine release from polynuclear basophils in whole blood of guinea pigs that had previously been sensitised. This test is used in drug safety, in support of the in vivo active systemic anaphylaxis test, which is sometimes required by regulatory authorities, when the observed clinical signs are not conclusive. This sensitive and discriminative test is inexpensive and reduces animal utilisation.


Anaphylaxis Basophils Histamine release In vitro Radioimmunoassay 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Gell PGH, Coombs RRA (1963) Clinical aspects of immunology. Blackwell Scientific Publications, Oxford, p 317Google Scholar
  2. Lagunoff D, Martin TW (1983) Agents that release histamine from mast cells. Ann Rev Pharmacol Toxicol 23:331–351Google Scholar
  3. Lichtenstein LM, McGlashan DW (1986) The concept of basophil releasability. J Allergy Clin Immunol 77:291–294Google Scholar
  4. Lichtenstein LM, Osler AG (1964) Studies on the mechanisms of hypersenitivity phenomena histamine release from human leucocytes by ragweed pollen antigen. J Exp Med 120:507–530Google Scholar
  5. McGlashan DW, Schleimer RP, Peters SP, Schulman ES, Adams GK, Sobotka AK et al. (1983) Comparative studies of human basophils and mast cells. Fed Proc 42:2504–2509Google Scholar
  6. Morel AM, Delage MA (1988) Immunoanalysis of histamine through a novel chemical derivatization. J Allergy Clin Immunol 82:646–654Google Scholar
  7. Motta I, Wong D (1969) Homologous and heterologous passive cutaneous anaphylactic activity of mouse antisera during the course of immunization. Life Sci 8:813–820Google Scholar
  8. Ovary Z (1964) Passive cutaneous anaphylaxis in CIOMS. In: Ackroyd JF (ed) Symposium on immunological method, Blackwell Scientific Publications, Oxford, pp 259–283Google Scholar
  9. Prieur DJ, Young DM, Davis RD, Cooney DA, Homan ER, Dixon RL et al. (1973) Procedures for preclinical toxicologic evaluation of cancer chemotherapeutic agents: protocols of the laboratory of toxicology. Cancer Chemother Rep 4:1–30Google Scholar
  10. Siraganian RP (1977) Automated histamine analysis for in vivo allergy testing. J Allergy Clin Immunol 59:214–222Google Scholar
  11. Siraganian RP (1983) Histamine secretion from mast cells and basophils. Trends Pharmacol Sci 4:432–437Google Scholar
  12. Straganian RP, Hook WA, Levine BB (1975) Specific in vitro histamine release for basophils by bivalent haptens; evidence for activation by simple bridging of membrane bound antibody. Immunochemistry 12:149–157Google Scholar
  13. Slater JE (1989) Rubber anaphylaxis. Lancet ii 1126–1130Google Scholar
  14. Tanizaki Y, Komagoe H, Morinaga H, Kitani H, Goda Y, Kimura I (1984) Allergen and anti IgE induced histamine release from whole blood. Int Arch Allergy Appl Immunol 73:141–145Google Scholar
  15. Wasserman SI (1983) Mediators of immediate hypersensitivity. J Allergy Clin Immunol 72:101–115Google Scholar

Copyright information

© Springer-Verlag 1992

Authors and Affiliations

  • D. Salauze
    • 1
  • A. Hery
    • 1
  • M. Corroller
    • 2
  1. 1.Rhone-Poulenc Rorer SADrug Safety Department; Clinical PathologyVitry CedexFrance
  2. 2.Rhone-Poulenc Rorer SADrug Safety Department; General ToxicologyVitry CedexFrance

Personalised recommendations