Comparative Haematology International

, Volume 2, Issue 3, pp 162–165 | Cite as

The radioimmunoassay of histamine release from circulating basophils in an in vitro study as support for the in vivo active systemic anaphylaxis test in the guinea pig

  • D. Salauze
  • A. Hery
  • M. Corroller
Original Articles
  • 25 Downloads

Abstract

An in vitro anaphylaxis test is described which explores the ability of compound re-challenge to induce histamine release from polynuclear basophils in whole blood of guinea pigs that had previously been sensitised. This test is used in drug safety, in support of the in vivo active systemic anaphylaxis test, which is sometimes required by regulatory authorities, when the observed clinical signs are not conclusive. This sensitive and discriminative test is inexpensive and reduces animal utilisation.

Keywords

Anaphylaxis Basophils Histamine release In vitro Radioimmunoassay 

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Copyright information

© Springer-Verlag 1992

Authors and Affiliations

  • D. Salauze
    • 1
  • A. Hery
    • 1
  • M. Corroller
    • 2
  1. 1.Rhone-Poulenc Rorer SADrug Safety Department; Clinical PathologyVitry CedexFrance
  2. 2.Rhone-Poulenc Rorer SADrug Safety Department; General ToxicologyVitry CedexFrance

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