Abstract
We describe a novel, PCR-based technique termed REVEAL-PCR for examining the inheritance of intracisternal A-type particles (IAP). Amplifications use an unlabeled primer to SINE repeats and a radiolabeled primer to the IAP long terminal repeat; labeled products, which can be resolved on sequencing gels, are formed when IAPs lie in proximity to SINEs. With this technique we have identified a total of 124 polymorphisms in the BXH and CXS recombinant inbred strains. We suggest that this method will be equally applicable for examining other gene families present at around a thousand copies per genome.
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Kaushik, N., Stoye, J.P. Intracisternal A-type particle elements as genetic markers: detection by repeat element viral element amplified locus-PCR. Mammalian Genome 5, 688–695 (1994). https://doi.org/10.1007/BF00426074
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DOI: https://doi.org/10.1007/BF00426074