Abstract
Lignobacter strain K17 is able to degrade aromatic compounds and to fix atmospheric nitrogen. It was proved that capacity for nitrogen fixation by Lignobacter was plasmid mediated. Plasmid pUCS100 (17.5 Mdal) carrying nif genes was transferred from Lignobacter to Escherichia coli SK1592 and Salmonella typhimurium. The transposon Tn9 was translocated to pUCS100 to facilitate selection of Nif+ bacteria. E. coli SK1592 harboring the new plasmid (pUCS101) reduced acetylene under anaerobic conditions. Plasmids pUCS100 and pUCS101 were not stably maintained in E. coli and S. typhimurium.
Similar content being viewed by others
Abbreviations
- Mdal:
-
megadalton
- CsCl-EtBr:
-
caesium chloride ethidium bromide
- m.o.i.:
-
multiplicity of infection
References
Cannon FC (1980) Genetics of nitrogen fixation. IV. International symposium programme and abstracts. Canberra, p 52
Cannon FC, Dixon RA, Postgate JR (1976) Derivation and properties of F-prime factor in Escherichia coli carrying nitrogen fixation genes from Klebsiella pneumoniae. J Gen Microbiol 93:111–125
Casse F, Boucher C, Julliot JS, Michel M, Dénarié J (1979) Identification and characterization of large plasmids in Rhizobium meliloti using agarose gel electrophoresis. J Gen Microbiol 113:229–242
Clewell DB (1969) Supercoiled circular DNA-protein complex in Escherichia coli: purification and induced conversion to an open circular DNA form. Proc Natl Acad Sci 62:1159–1166
Cohen SN, Chang ACY, Hsu L (1972) Nonchromosomal antibiotic resistance in bacteria: genetic transformation of Escherichia coli by R-factor DNA. Proc Natl Acad Sci 69:2110–2114
Davis BD, Minigioli ES (1950) Mutants of Eschericia coli requiring methionine or vitamin B12. J Bacteriol 60:17–25
Derylo M, Skorupska A, Lorkiewicz Z (1978) Detection of small cryptic plasmids in Salmonella typhimurium strain LT2. Arch Microbiol 119:87–90
Dixon R, Cannon FC, Kondorosi A (1976) Construction of a plasmid carrying nitrogen fixation genes from Klebsiella pneumoniae. Nature 260:268–271
Dixon R, Eady RR, Espin G, Hill S, Iaccarino M, Kahn D, Merrick M (1980) Analysis of regulation of Klebsiella pneumoniae nitrogen fixation (nif) gene cluster with gene fusions. Nature 286:128–132
Helling RB, Goodman HM, Boyer HW (1974) Analysis of endonuclease R EcoRI fragments of DNA from lambdoid bacteriophages and other viruses by agarose-gel electrophoresis. J Virology 14:1235–1244
Hughes C, Meynell GG (1977) Rapid screening for plasmid DNA. Molec Gen Genet 151:175–179
Kleckner N (1977) Translocatable elements in procaryotes. Cell 11:11–23
Krishnapillai V, Postgate JR (1980) Expression of Klebsiella his and nif genes in Serratia marcescens, Erwinia herbicola and Proteus mirabilis. Arch Microbiol 127:115–118
MacNeil T, MacNeil D, Roberts GP, Supiano M, Brill WJ (1978) Fine structure mapping and complementation analysis of nif (nitrogen fixation) genes in Klebsiella pneumoniae. J Bacteriol 136:253–266
Postgate JR, Krishnapillai V (1979) Expression of Klebsiella nif and his genes in Salmonella typhimurium. J Gen Microbiol 98:379–385
Rapin AMC, Kalckar HM (1971) The relation of bacteriophage attachment to lipopolysaccharide structure. In: Weinbaum G, Kadis S, Ail SJ (eds) Microbial toxins, Vol 4. Academic Press, New York London, pp 267–301
Riedel GE, Ausubel FM, Cannon FC (1979) Physical map of chromosomal nitrogen fixation (nif) genes of Klebsiella pneumoniae. Proc Natl Acad Sci 76:2866–2870
Roberts GP, MacNeil T, MacNeil D, Brill W (1978) Regulation and characterization of protein products coded by the nif (nitrogen fixation) genes of Klebsiella pneumoniae. J Bacteriol 136:267–279
Salkinoja-Salonen MS, Vaisanen E, Peterson A (1979) Involvement of plasmids in the bacterial degradation of lignin-derived compounds. In: Timmis KN, Pühler A (eds) Plasmids of medical, environmental and commercial importance. Elsevier/North Holland Biomedical Press, Amsterdam New York Oxford, pp. 301–314
Yensen V, Holm E (1975) Associative growth of nitrogen fixing bacteria with other microorganisms. In: Stewart WDP (ed) Nitrogen fixation by free living microorganisms. Cambridge University Press, Cambridge, pp 101–119
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Derylo, M., Glowacka, M., Skorupska, A. et al. Nif plasmid from Lignobacter . Arch. Microbiol. 130, 322–324 (1981). https://doi.org/10.1007/BF00425948
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00425948