Molecular and General Genetics MGG

, Volume 180, Issue 2, pp 405–410 | Cite as

Transposition of Tn 1 to the Rhizobium meliloti genome

  • Josep Casadesús
  • Enrique Iáñez
  • José Olivares
Article

Summary

A derivative of the IncP1 plasmid RP4, carrying the thermoinducible prophage Mucts62, was obtained in Escherichia coli K 12 J53 (RP4). It was impossible to maintain the hybrid plasmid RP4: Mucts62 in Rhizobium meliloti GR4. Thus, it was used as a vehicle for introducing the ampicillinresistant transposon Tn1 introducing the ampicillinresistant transposon Tn1 into the R. meliloti genome.

Transposition of Tn1 did not generate auxotrophic strains, suggesting that the insertion of Tn1 into the R. meliloti genome was relatively specific. Two chromosomal hot spots for Tn1 insertion were identified by cotransductional analysis, after general transduction by phage DF2. Plasmid-curing experiments, carried out by heat treatment, revealed that symbiotic plasmid(s) also contain at least one site for Tn1 insertion.

Keywords

Escherichia Coli Heat Treatment Rhizobium General Transduction Auxotrophic Strain 

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Copyright information

© Springer-Verlag 1980

Authors and Affiliations

  • Josep Casadesús
    • 1
  • Enrique Iáñez
    • 2
  • José Olivares
    • 2
  1. 1.Departmento de Microbiologiá, Facultad de FarmaciaUniversidad de GranadaGranadaSpain
  2. 2.Departamento de Microbiologiá, Estación Experimental del ZaidinCSICGranadaSpain

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