Summary
A gene coding for a modifying DNA-methyltransferase which methylates the central C in the BsuR recognition sequence 5′GGCC was identified in the genome of the temperate Bacillus subtilis phage SPβ. This gene is expressed only after induction of the prophage by either mitomycin C or UV. The presence of active methyltransferase in induced cells leads to modification of BsuR recognition sites in SPβ DNA as well as in heterologous DNA.
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Communicated by W. Arber
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Trautner, T.A., Pawlek, B., Günthert, U. et al. Restriction and modification in Bacillus subtilis: Identification of a gene in the temperate phage SPβ coding for a BsuR specific modification methyltransferase. Molec. Gen. Genet. 180, 361–367 (1980). https://doi.org/10.1007/BF00425849
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DOI: https://doi.org/10.1007/BF00425849