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Control of sporulation in yeast: SAD1-a mating-type specific, unstable alteration that uncouples sporulation from mating-type control

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Summary

Normally, in order to sporulate, a diploid yeast cell must be heterozygous (MAT a/MATα) at the mating-type locus. In a new mutant, this requirement is circumvented by SAD1. This alteration is mating-type specific; it allows sporulation of MATα/ MATα. MATα/mat a-1, and MATα/matα-2 diploid cells, but not MAT a/MAT a or MAT a/0 (monosomic) strains. Other than acquiring the ability to sporulate, SAD1 cells behave as wild-type MATα/MATα strains; they exhibit medial budding, normal mating factor production and response and mate with normal mating efficiencies and kinetics. The segregation of SAD1 is often bizarre; for example, MATα/MATα strains which were constructed to be heterozygous SAD1/+ often segregate 4 SAD1:0+progeny and strains which were constructed to be homozygous SAD1/ SAD1 sometimes segregated 1 SAD1:3+progeny. The genetic analyses of SAD1 suggest that it is dominant and is located ∼30 cM from MAT on chromosome III.

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Authors and Affiliations

  1. Department of Biological Chemistry, The Milton S. Hershey Medical Center, The pennsylvania State University, 17033, Hershey, Pennsylvania, USA

    Anita K. Hopper

  2. Walsman Institute of Microbiology, Rutgers University, P.O. Box 759, 08854, Piscataway, New Jersey, USA

    Vivian L. MacKay

Authors
  1. Anita K. Hopper
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  2. Vivian L. MacKay
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Communicated by G.R. Fink

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Hopper, A.K., MacKay, V.L. Control of sporulation in yeast: SAD1-a mating-type specific, unstable alteration that uncouples sporulation from mating-type control. Molec. Gen. Genet. 180, 301–314 (1980). https://doi.org/10.1007/BF00425842

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  • DOI: https://doi.org/10.1007/BF00425842

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