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Molecular cloning, sequencing and sequence analysis of the fox-2 gene of Neurospora crassa encoding the multifunctional β-oxidation protein

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Abstract

We present the molecular cloning and sequencing of genomic and cDNA clones of the fox-2 gene of Neurospora crassa, encoding the multifunctional β-oxidation protein (MFP). The coding region of the fox-2 gene is interrupted by three introns, one of which appears to be inefficiently spliced out. The encoded protein comprises 894 amino acid residues and exhibits 45% and 47% sequence identity with the MFPs of Candida tropicalis and Saccharomyces cerevisiae, respectively. Sequence analysis identifies three regions of the fungal MFPs that are highly conserved. These regions are separated by two segments that resemble linkers between domains of other MFPs, suggesting a three-domain structure. The first and second conserved regions of each MFP are homologous to each other and to members of the short-chain alcohol dehydrogenase family. We discuss these homologies in view of recent findings that fungal MFPs contain enoyl-CoA hydratase 2 and d-3-hydroxyacyl-CoA dehydrogenase activities, converting trans-2-enoyl-CoA via d-3-hydroxyacyl-CoA to 3-ketoacyl-CoA. In contrast to its counterparts in yeasts, the Neurospora MFP does not have a C-terminal sequence resembling the SKL motif involved in protein targeting to microbodies.

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Authors and Affiliations

  1. Institut für Physiologische Chemie, Abteilung für Zellbiochemie, Medizinische Fakultät, Ruhr-Universität Bochum, Postfach 102148, D-44780, Bochum, Germany

    Alexander Fosså, Andreas Beyer, Edith Pfitzner, Bettina Wenzel & Wolf -H. Kunau

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  1. Alexander Fosså
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  2. Andreas Beyer
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  3. Edith Pfitzner
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  4. Bettina Wenzel
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  5. Wolf -H. Kunau
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Additional information

Communicated by C. Hollenberg

The first two authors have contributed equally to this paper

The nucleotide sequence reported in this paper has been submitted to the GenBank/EMBL Data Bank with accession number 80052

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Fosså, A., Beyer, A., Pfitzner, E. et al. Molecular cloning, sequencing and sequence analysis of the fox-2 gene of Neurospora crassa encoding the multifunctional β-oxidation protein. Molec. Gen. Genet. 247, 95–104 (1995). https://doi.org/10.1007/BF00425825

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