Archives of Microbiology

, Volume 137, Issue 1, pp 3–9 | Cite as

Gluconate catabolism in cowpea rhizobia: evidence for a ketogluconate pathway

  • Mark D. Stowers
  • Gerald H. Elkan
Original Papers

Abstract

Enzymatic and radiorespirometric analysis of several strains of cowpea rhizobia revealed the presence of key enzymes of the Entner-Doudoroff (ED) pathway with the operation of the hexose cycle for the dissimilation of gluconate. These bacteria lack the oxidative pentose phosphate (PP) pathway when grown on gluconate. Gluconate-grown cells possessed an operational tricarboxylic acid (TAC) cycle. Enzymes of an ancillary pathway, the ketogluconate (KG) pathway for gluconate catabolism were detected. The presence of this pathway was confirmed by techniques of thin-layer chromatography and radiorespirometry.

Key words

Rhizobium-Rhizobium sp. 32H1-cowpea rhizobia Gluconate catabolism Ketogluconate pathway 

Abbreviations

ED

Entner Doudoroff

PP

pentose phosphate

EMP

Embden-Meyerhof-Parnas

KG

ketogluconate

TCA

tricarboxylic acid

DKG

diketogluconate

PFK

phosphofructokinase

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Copyright information

© Springer-Verlag 1984

Authors and Affiliations

  • Mark D. Stowers
    • 1
  • Gerald H. Elkan
    • 2
  1. 1.NPIUniversity Research ParkSalt Lake CityUSA
  2. 2.Department of MicrobiologyNorth Carolina State UniversityRaleighUSA

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