Summary
In ‘developmental mutants’ of staminogenesis carrying sensitive cytoplasm (male sterile strains of the dioecious plant Mercurialis annua), the increasing levels of tRNAs tyr aminoacylation as a function of the degree of sterility confirm the classification of strains previously obtained by means of genetics and physiology: the series begins with fertile males with normal cytoplasm (0.61), restored fertiles (0.74), semisteriles (0.74) and total steriles (0.84), (all with S cytoplasm) and ends with normal females (1.06). The fractionation of tyrosyl-tRNAs shows that two chloroplast tyrosine tRNAs isoacceptors absent in normal fertile staminogenesis and present in female organogenesis are always expressed in strains with S cytoplasm, in the presence or absence of fertility nuclear restorer genes. In S cytoplasm the chloroplast escapes from its normal regulation during sexual differentiation. In restored fertile males (S cytoplasm), the nuclear restorer genes do not suppress but only compensate the chloroplast disfunction that remains of female type.
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Louis, J.P. Sexual differentiation in Mercurialis annua L. (2n=16): Evidence for a control system of nucleo-cytoplasmic relationships by the study of tyrosyl-tRNAs from ‘developmental mutants’ of male sterility. Molec. Gen. Genet. 191, 473–479 (1983). https://doi.org/10.1007/BF00425765
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DOI: https://doi.org/10.1007/BF00425765