Summary
The product of the uvrD gene of Escherichia coli is involved in the repair of DNA damage, mismatch repair, and recombination. Phage Mud(Amp, Lac) was used to from a uvrD-lacZ fusion allowing uvrD expression to be followed by measuring the activity of β-galactosidase, the product of the lacZ gene. uvrD expression was inducible by DNA damage and was under the control of lexA-recA regulatory system. Mutations in the uvrD gene that result in different phenotypes in respect to DNA repair and spontaneous mutation have been previously found. The phenotype of the uvrD::Mud(Amp, Lac) mutant was mutator and UV-sensitive but not as deficient in host cell reactivation or repair of methyl methanesulfonate damage as the previously described uvrD3 mutant.
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Communicated by P.T. Emmerson
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Siegel, E.C. The Escherichia coli uvrD gene is inducible by DNA damage. Molec. Gen. Genet. 191, 397–400 (1983). https://doi.org/10.1007/BF00425753
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DOI: https://doi.org/10.1007/BF00425753