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Molecular and General Genetics MGG

, Volume 191, Issue 3, pp 347–352 | Cite as

Molecular characterization of ilvC specialized transducing phages of Escherichia coli K-12

  • Norman W. Baylor
  • Arthur L. Williams
  • Nathan Cofie
Article
  • 32 Downloads

Summary

A series of lambda defective ilvC specialized transducing phage has been isolated which carry regions of isoleucine and valine structural and regulatory genes derived from the ilv cluster at minute 83 on the linkage map of the chromosome of Escherichia coli K-12. The ilv genes carried by these phages and their order have been determined by transduction of auxotrophs. The ilvC+ lysogen of an ilvC- strain gave rise, after heat induction of the lysogen, to transducing particles which carried the wild-type allele of the cya-marker. Further experiments have shown that the lambda defective ilvC phages were able to cotransduce a rho-15ts mutation as well as a rep-5 mutation. Hence, the order of the clockwise excision of the ilv cluster was found to be ilvC-rho-rep-cya. Enzyme levels in strains carrying the lambda defective ilvC phages indicated that the ilvC gene was not altered by the insertion of lambda into the ilv cluster. The isolation and digestion of lambda defective ilvC DNA by EcoRI and HindIII restriction endonucleases demonstrated that the specialized transducing phages carried part of the genome from the E. coli K-12 chromosome.

Keywords

Enzyme Escherichia Coli Regulatory Gene Restriction Endonuclease Valine 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1983

Authors and Affiliations

  • Norman W. Baylor
    • 1
  • Arthur L. Williams
    • 1
  • Nathan Cofie
    • 1
  1. 1.School of Biological SciencesUniversity of KentuckyLexingtonUSA

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