Molecular and General Genetics MGG

, Volume 191, Issue 3, pp 339–346 | Cite as

An insertion mutation associated with constitutive expression of repressible acid phosphatase in Saccharomyces cerevisiae

  • Akio Toh-e
  • Yoshinobu Kaneko
  • Jirô Akimaru
  • Yasuji Oshima
Article

Summary

The PHO83 mutation in Saccharomyces cerevisiae, which had been detected on the basis of constitutive production of repressible acid phosphatase and mapped at the end of the PHO5 locus, was analysed by Southern hybridization with cloned DNA fragments of the PHO5 gene as probe. It was shown that this mutant has a DNA insertion of about 6 kilobase pairs, probably in the 5′-noncoding region of the PHO5 gene. Production of repressible acid phosphatase by the PHO83 mutant is partially independent of the function of the PHO2 and PHO4 genes, the positive regulatory genes whose functions are indispensable for PHO5 expression. PHO83 mutants are constitutive in a or α cells, either haploid or diploid, but not in non-mating cells, MATa/MATα or a certain sterile mutation. These observations strongly suggest that the PHO83 mutation is caused by insertion of a Ty element in the 5′-noncoding region of the PHO5 gene.

Keywords

Regulatory Gene Saccharomyces Cerevisiae Saccharomyces Acid Phosphatase Constitutive Expression 

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Copyright information

© Springer-Verlag 1983

Authors and Affiliations

  • Akio Toh-e
    • 1
  • Yoshinobu Kaneko
    • 2
  • Jirô Akimaru
    • 2
  • Yasuji Oshima
    • 2
  1. 1.Department of Fermentation TechnologyHiroshima UniversityHigashihiroshima-shi, Hiroshima
  2. 2.Department of Fermentation TechnologyOsaka UniversitySuita-shi, OsakaJapan

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