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Molecular and General Genetics MGG

, Volume 200, Issue 3, pp 472–475 | Cite as

Cellular localization and export of the soluble haemolysin of Vibrio cholerae El Tor

  • Antonietta Mercurio
  • Paul A. Manning
Article

Summary

The cellular location of the haemolysin of Vibrio cholerae El Tor strain 017 has been analyzed. This protein is found both in the periplasmic space and the extracellular medium in Vibrio cholerae. However, when the cloned gene, present on plasmid pPM431, is introduced into E. coli K-12 this protein remains localized predominantly in the periplasmic space with no activity detected in the extracellular medium. Mutants of E. coli K-12 (tolA and tolB) which leak periplasmic proteins mimic excretion and release the haemolysin into the growth medium. Secretion of haemolysin into the periplasm is independent of perA (envZ) and in fact, mutants in perA (envZ) harbouring pPM431 show hyperproduction of periplasmic haemolysin. These results in conjunction with those for other V. cholerae extracellular proteins suggest that although E. coli K-12 can secrete these proteins into the periplasm, it lacks a specific excretion mechanism, present in V. cholerae, for the release of soluble proteins into the growth medium.

Keywords

Growth Medium Soluble Protein Vibrio Cellular Localization Extracellular Protein 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1985

Authors and Affiliations

  • Antonietta Mercurio
    • 1
  • Paul A. Manning
    • 1
  1. 1.Department of Microbiology and ImmunologyThe University of AdelaideAdelaideAustralia

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