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Molecular and General Genetics MGG

, Volume 200, Issue 3, pp 439–441 | Cite as

Evidence that a system similar to the recA system of Escherichia coli exists in Vibrio cholerae

  • R. K. Ghosh
  • K. A. I. Siddiqui
  • G. Mukhopadhyay
  • Amit Ghosh
Article

Summary

Two lines of evidence suggest that a gene analogous to the recA gene of Escherichia coli exists in Vibrio cholerae and that its product serves a proteolytic function in the SOS response. Firstly, Southern blot hybridization using the recA gene of E. coli as a probe revealed a genomic sequence in V. cholerae which hybridized with the probe. Secondly, the SOS-like response in V. cholerae (as measured by beta phage induction) triggered by DNA damaging agents like Furazolidone could be blocked by Antipain, a protease inhibitor known to inhibit RecA protease action in E. coli. Maximal blocking effect of Antipain on beta phage induction occurred at 1 mM. At this concentration neither the viability of the host bacterium nor the lytic growth of a clear plaque mutant of the phage was affected by Antipain.

Keywords

Vibrio Blot Hybridization Blocking Effect recA Gene Southern Blot Hybridization 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1985

Authors and Affiliations

  • R. K. Ghosh
    • 1
  • K. A. I. Siddiqui
    • 1
  • G. Mukhopadhyay
    • 1
  • Amit Ghosh
    • 1
  1. 1.Indian Institute of Chemical BiologyJadavpurIndia

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