Summary
Two lines of evidence suggest that a gene analogous to the recA gene of Escherichia coli exists in Vibrio cholerae and that its product serves a proteolytic function in the SOS response. Firstly, Southern blot hybridization using the recA gene of E. coli as a probe revealed a genomic sequence in V. cholerae which hybridized with the probe. Secondly, the SOS-like response in V. cholerae (as measured by beta phage induction) triggered by DNA damaging agents like Furazolidone could be blocked by Antipain, a protease inhibitor known to inhibit RecA protease action in E. coli. Maximal blocking effect of Antipain on beta phage induction occurred at 1 mM. At this concentration neither the viability of the host bacterium nor the lytic growth of a clear plaque mutant of the phage was affected by Antipain.
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Communicated by H. Böhme
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Ghosh, R.K., Siddiqui, K.A.I., Mukhopadhyay, G. et al. Evidence that a system similar to the recA system of Escherichia coli exists in Vibrio cholerae . Molec. Gen. Genet. 200, 439–441 (1985). https://doi.org/10.1007/BF00425728
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DOI: https://doi.org/10.1007/BF00425728