Molecular and General Genetics MGG

, Volume 200, Issue 3, pp 439–441 | Cite as

Evidence that a system similar to the recA system of Escherichia coli exists in Vibrio cholerae

  • R. K. Ghosh
  • K. A. I. Siddiqui
  • G. Mukhopadhyay
  • Amit Ghosh


Two lines of evidence suggest that a gene analogous to the recA gene of Escherichia coli exists in Vibrio cholerae and that its product serves a proteolytic function in the SOS response. Firstly, Southern blot hybridization using the recA gene of E. coli as a probe revealed a genomic sequence in V. cholerae which hybridized with the probe. Secondly, the SOS-like response in V. cholerae (as measured by beta phage induction) triggered by DNA damaging agents like Furazolidone could be blocked by Antipain, a protease inhibitor known to inhibit RecA protease action in E. coli. Maximal blocking effect of Antipain on beta phage induction occurred at 1 mM. At this concentration neither the viability of the host bacterium nor the lytic growth of a clear plaque mutant of the phage was affected by Antipain.


Vibrio Blot Hybridization Blocking Effect recA Gene Southern Blot Hybridization 
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  1. Denhardt DT (1966) A membrane filter technique for the detection of complementary DNA. Biochem Biophys Res Commun 23:641–646Google Scholar
  2. Hyman RW, Brunovskis I, Summers WC (1973) DNA base sequence homology between coliphages T7 & ø II and between T3 and ø II as determined by heteroduplex mapping in the electron microscope. J Mol Biol 77: 189–196Google Scholar
  3. Lytle CD, Goddard JG, Buchta FL (1978) Protease inhibitors prevent UV-enhanced virus reactivation in E. coli but not in monkey kidney cells. In: Hanawalt PC, Friedberg EC, Fox CF (eds) DNA repair mechanisms. Academic Press, New York, pp 559–562Google Scholar
  4. Mekalanos JJ (1983) Duplication and amplification of toxin genes in Vibrio cholerae. Cell 35:253–263Google Scholar
  5. Meyn MS, Rossman T, Troll W (1977) A protease inhibitor blocks SOS function in Escherichia coli: Antipain prevents repressor inactivation, ultraviolet mutagenesis and filamentous growth. Proc Natl Acad Sci USA 74:1152–1156Google Scholar
  6. Meyn MS, Rossman T, Gottlieb P, Troll W (1978) The role of protease in SOS regulation. In: Hanawalt PC, Friedberg EC, Fox CF (eds) DNA repair mechanisms. Academic Press, New York, pp 379–382Google Scholar
  7. Michel S, Laval F (1982) Protease inhibitors suppress the survival increase mediated by uncouplers in X-irradiated mammalian cells. Biochemie 64:753–755Google Scholar
  8. Miller JH (1972) Determination of viable cell counts: Bacterial growth curves. In: Experiments in molecular genetics. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, pp 31–36Google Scholar
  9. Rigby PW, Dieckmann M, Rhodes C, Berg P (1977) Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase. I. J Mol Biol 113:237–251Google Scholar
  10. Sancar A, Rupp WD (1979) Physical map of the recA gene. Proc Natl Acad Sci USA 76:3144–3148Google Scholar
  11. Sancar A, Stachelek C, Konigsberg W, Rupp WD (1980) Sequence of the recA gene and protein. Proc Natl Acad Sci USA 77:2611–2615Google Scholar
  12. Siddiqui KAI, Ghosh A (1983) Temperate phage induction and filament formation in Vibrio cholerae by Furazolidone. Biochem Biophys Res Comm 112:1106–1111Google Scholar
  13. Southern EM (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 98:503–517Google Scholar
  14. Swenson PA (1979) Antipain lethality to Escherichia coli: Dependence upon cyclic adenosine 3′, 5′ monophosphate and its receptor protein. J Bacteriol 139:690–693Google Scholar
  15. Umezawa H (1976) Structures and activities of protease inhibitors of microbial origin. Methods Enzymol 45:678–695Google Scholar
  16. Walker GC (1984) Mutagenesis and inducible esponses to deoxyribonucleic acid damage in Escherichia coli. Microbiol Rev 48:60–93Google Scholar
  17. Yang RCA, Wu R (1979) BK virus DNA sequence: Extent of homology with simian virus 40 DNA. Proc Natl Acad Sci USA 76:1179–1183Google Scholar

Copyright information

© Springer-Verlag 1985

Authors and Affiliations

  • R. K. Ghosh
    • 1
  • K. A. I. Siddiqui
    • 1
  • G. Mukhopadhyay
    • 1
  • Amit Ghosh
    • 1
  1. 1.Indian Institute of Chemical BiologyJadavpurIndia

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