Molecular and General Genetics MGG

, Volume 201, Issue 2, pp 192–197 | Cite as

Structure of an amplifiable DNA sequence in Streptomyces lividans 66

  • Josef Altenbuchner
  • John Cullum
Article

Summary

Spontaneous chloramphenicol-sensitive mutants of Streptomyces lividans 66 had previously been shown to be very unstable and to yield arginine auxotrophic mutants at a frequency of 25% of spores; the Arg- mutants had amplified a particular 5.7 kb DNA sequence to over one hundred tandem copies per genome. In this paper we report the cloning of the amplifiable region from amplified and wild-type strains. This showed that the amplifiable fragment is already present as a duplication in wild type cells. Hybridisation experiments also demonstrated that in the amplified strains there was a deletion of neighbouring DNA sequences to one side of the amplifiable element; sequences to the other side remain intact.

Keywords

Arginine Streptomyces Wild Type Cell Hybridisation Experiment Auxotrophic Mutant 

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Copyright information

© Springer-Verlag 1985

Authors and Affiliations

  • Josef Altenbuchner
    • 1
  • John Cullum
    • 1
  1. 1.Department of Biochemistry and Applied Molecular BiologyU.M.I.S.T.ManchesterUK

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