Molecular and General Genetics MGG

, Volume 142, Issue 3, pp 185–191 | Cite as

Restriction and modification in B. subtilis

The biochemical basis of modification against endo R · Bsu R restriction
  • U. Günthert
  • J. Stutz
  • G. Klotz
Article

Summary

The content of 5-methylcytosine (5MC) and 6-methyladenine (6MA) in modified and nonmodified DNAs from B. subtilis and B. subtilis phage SPP1 were determined. Nonmodified SPP1 · O DNA contains about 15 5MC residues/molecule. Each modified SPP1 ·R DNA molecule carries 190 modification specific methyl groups. This number is sufficient to account for modification of the 80 restriction sites in SPP1 DNA (Bron and Murray, 1975) against endo R · Bsu R, assuming each modified site contains two 5MC residues. Resistance of SP01 DNA against endo R · Bsu R restriction both in vivo and in vitro is probably not due to methylation of endo R·Bsu R recognition sites.

Keywords

Methyl Restriction Site Recognition Site Specific Methyl Subtilis Phage 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Boyer, H. W.: Restriction and modification of DNA: Enzymes and substrates. Fed. Proc. 33, 1125–1127 (1974)Google Scholar
  2. Bron, S., Murray, K.: Restriction and Modification in B. subtilis. Nucleotide sequence recognized by restriction endonuclease R · Bsu R from strain R. Molec. gen. Genet. (in press) 1975Google Scholar
  3. Bron, S., Murray, K., Trautner, T. A.: Restriction and Modification in B. subtilis. Purification and general properties of a restriction endonuclease from strain R. Molec. gen. Genet. (in press) 1975Google Scholar
  4. Esche, H., Schweiger, M., Trautner, T. A.: Gene expression of bacteriophage SPP1: I. Phage directed protein synthesis. Molec. gen. Genet. (in press) 1975Google Scholar
  5. Fink, R. M., Fink, K.: Relative retention of 3H and 14C-labels of nucleotides incorporated into nucleic acids of neurospora. J. biol. Chem. 237, 2889–2891 (1962)Google Scholar
  6. Gough, M., Lederberg, S.: Methylated bases in the host-modified DNA of E. coli and bacteriophage λ. J. Bact. 91, 1460–1468 (1966)Google Scholar
  7. Günthert, U.: DNA-Methylierung während der Entwicklung von Pro- und Eukaryonten. Ph. D. Thesis, Tübingen, 1975Google Scholar
  8. Kappler, J. W.: The 5MC content of DNA: Tissue specificity. J. cell. Physiol. 78, 33–36 (1971)Google Scholar
  9. Mamelak, L., Boyer, H. W.: Genetic control of the secondary modification of DNA in E. coli. J. Bact. 104, 57–62 (1970)Google Scholar
  10. Marinus, M. G., Morris, N. R.: Biological function for 6MA residues in the DNA of E. coli K12. J. molec. Biol. 85, 309–322 (1974)Google Scholar
  11. Okubo, S., Strauss, B., Stodolsky, M.: The possible role of recombination in the infection of competent B. subtilis by bacteriophage DNA. Virology 24, 552–562 (1964)Google Scholar
  12. Riva, S., Polsinelli, M., Falaschi, A.: A new phage of B. subtilis with infectious DNA having separable strands. J. molec. Biol. 35, 347–356 (1968)Google Scholar
  13. Smith, H. O., Nathans, D.: A suggested nomenclature for bacterial host modification and restriction systems and their enzymes. J. molec. Biol 81, 419–423 (1973)Google Scholar
  14. Smith, J. D., Arber, W., Kühnlein, U.: Host specificity of DNA produced by E. coli. XIV. The role of nucleotide methylation in in vivo B-specific modification. J. molec. Biol. 63, 1–8 (1972)Google Scholar
  15. Spatz, H. Ch., Trautner, T. A.: One way to do experiments on gene conversion? Molec. gen. Genet. 109, 84–106 (1970)Google Scholar
  16. Spatz, H. Ch., Trautner, T. A.: One role of recombination in transfection of B. subtilis. Molec. gen. Genet. 113, 174–190 (1971)Google Scholar
  17. Spizizen, J.: Transformation of biochemically deficient strains of Bacillus subtilis by deoxyribonucleate. Proc. nat. Acad. Sci. Wash. 44, 1072–1078 (1958)Google Scholar
  18. Trautner, T. A., Pawlek, B., Bron, S., Anagnostopoulos, C.: Restriction and modification in B. subtilis. Biological Aspects. Molec. gen. Genet. 131, 181–191 (1974)Google Scholar
  19. Wyatt, G. R., Cohen, S. S.: The bases of the nucleic acids of some bacterial and animal viruses: The occurrence of 5-hydroxymethylcytosine. Biochem. J. 55, 774–782 (1973)Google Scholar

Copyright information

© Springer-Verlag 1975

Authors and Affiliations

  • U. Günthert
    • 1
  • J. Stutz
    • 1
  • G. Klotz
    • 1
  1. 1.Abt. TrautnerMax-Planck-Institut für molekulare genetikBerlinGermany

Personalised recommendations