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Chromosome replication following a temporary inhibition of DNA-synthesis by nalidixic acid in a temperature-sensitive dnaA mutant of Escherichia coli

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Summary

Chromosome replication cycle in a DNA initiation mutant of Escherichia coli (CRT-83, dnaAts) was blocked by nalidixic acid, an inhibitor of the A subunit of DNA gyrase. Following a period of inhibition of DNA synthesis, the drug was removed and “run-out” DNA synthesis was examined. It was found that the “capacity” for DNA synthesis was not affected by such a treatment.

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Authors and Affiliations

  1. Department of Microbiology, College of Medicine, University of Saskatchewan, S7N OWO, Saskatoon, Canada

    G. G. Khachatourians

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  1. G. G. Khachatourians
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Communicated by G.O'Donovan

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Khachatourians, G.G. Chromosome replication following a temporary inhibition of DNA-synthesis by nalidixic acid in a temperature-sensitive dnaA mutant of Escherichia coli . Molec. Gen. Genet. 181, 411–413 (1981). https://doi.org/10.1007/BF00425623

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  • DOI: https://doi.org/10.1007/BF00425623

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