Summary
The structure and formation of polyphosphate-granules has been investigated by means of ultrathin sections through Mycobacterium phlei.
After removing the polyphosphate, a fine grained and scarcely electron-scattering structure was to be seen which, however, was not bordered by a membrane.
The granules probably originate in distinct regions of the cytoplasm from irregularly shaped intermediate stages or from agglomerations of micro-granules.
Mitochondria-like structures of Mycobacterium phlei are described. Polyphosphates probably may be accumulated in these structures as well.
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Literatur
Bassermann, F. J.: Struktur des Tuberkuloseerregers in ultradünnen Schnitten nach Fixierung bei verschiedenen pH-Werten. Z. Naturforsch. 11b, 276 (1956).
Beloserski, A. N.: Polyphosphates, their formation and significance for the development of certain lower organisms. 4. Int. Kongr. Biochem. Wien 1958, Ref. 3-12.
Drews, G.: Die granulären Einschlüsse der Mycobakterien. Arch. Mikrobiol. 28, 369 (1958).
: Die Bildung metachromatischer Granula in wachsenden und ruhenden Kulturen von Mycobacterium phlei. Naturwissenschaften 46, 87 (1959).
Knaysi, G.: On the nature of the granules of Mycobacteria. J. Bact. 74, 12 (1957).
Knöll, H., u. W. Niklowitz: Zur Feinstruktur der Sarcina ventriculi. Arch. Mikrobiol. 31, 125 (1958).
Kölberl, H.: Untersuchungen am Mycobacterium tuberculosis. III. Mitt. Z. Naturforsch. 10b, 433 (1955).
: Über einige Probleme der Morphologie und Cytologie des Mycobacterium tuberculosis. Jber. Borstel 4, 252 (1956/57).
Kölbel, H.: Untersuchungen am Mycobact. tub., 5. Mitt. Zbl. Bakt. I. Abt. Orig. 171, 486 (1958).
Krüger-Thiemer, E., u. A. Lembke: Zur Definition der Mycobakteriengranula. Naturwissenschaften 41, 146–147 (1954).
Langen, P., u. E. Liss: Über Bildung und Umsatz der Polyphosphate der Hefe. Biochem. Z. 330, 455 (1958).
Massey, B. W.: Ultrathin sectioning for electron microscopy. Stain Technol. 28, 19 (1953).
Mudd, S., L. C. Winterscheid, E. De Lamater and H. Henderson: Evidence suggesting that the granules of mycobacteria are mitochondria. J. Bact. 62, 459 (1951).
Mudd, S., K. Takeya and H. J. Henderson: Electron-scattering granules and reducing sites in Mycobacteria. J. Bact. 72, 767 (1956).
Mudd, S., A. Yoshida and M. Koike: Polyphosphate as accumulator of phosphorus and energy. J. Bact. 75, 224 (1958).
Niklowitz, W.: Über die Herstellung von Ultradünnschnitten mit einer Uhrmacherdrehbank. Mikroskopie 10, 401 (1955).
Niklowitz, W., u. G. Drews: Zur elektronenmikroskopischen Darstellung der Feinstruktur von Rhodospirillum rubrum. Arch. Mikrobiol. 23, 123 (1955).
Niklowitz, W.: Mitochondrienäquivalente bei E. coli. Zbl. Bakt., I. Abt. Orig. 173, 12 (1958).
Ruska, H., G. Bringmann, I. Neckel u. G. Schuster: Über die Entwicklung sowie den morphologischen und cytologischen Aufbau von Mycobact. avium. Z. wiss. Mikr. 60, 425 (1952).
Shinohara, C.: An electron microscop study of tubercle bacilli. I. Sci. Rep. Res. Inst. Tohoku Univ. (Japan) 6, 1 (1955).
Shinohara, C., K. Fukushi, J. Suzuki and K. Sato: Mitochondrial structure of Mycobact. tuberculosis. J. Electronmicroscopy 6, 47 (1958).
Weinreb, S.: Ultraviolet polymerization of monomeric metacrylates for electron microscopy. Science 121, 774 (1955).
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Drews, G. Elektronenmikroskopische Untersuchungen an Mycobacterium phlei. Archiv. Mikrobiol. 35, 53–62 (1960). https://doi.org/10.1007/BF00425595
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DOI: https://doi.org/10.1007/BF00425595