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Archives of Microbiology

, Volume 149, Issue 5, pp 466–470 | Cite as

Transport of leucine in the cyanobacterium Anabaena variabilis

  • Teresa Thiel
Original Papers

Abstract

The amino acid leucine was transported by the cyanobacterium Anabaena variabilis. The Km for transport was 10.8 μM; the Vmax was 8.7 nmoles min−1 mg−1 chlorophyll a. Transport of leucine was energy dependent: uptake of leucine was inhibited in the dark, and by DCMU and cyanide. Transport was neither dependent on nor enhanced by Na+. Prior growth of cells with leucine did not repress transport of [14C]-leucine. Alanine, glycine, valine, and methionine were strong competitive inhibitors of leucine uptake; serine, threonine, isoleucine, norleucine, and d-alanine competitively inhibited to a lesser degree. Other amino acids or amino acid analogues, including d-leucine, α-aminoisobutyrate, and d-serine did not inhibit the transport of leucine.

Key words

Amino acid transport Leucine Threonine Cyanobacteria Blue-green algae 

Abbreviations

Chl a

chlorophyll a

DCMU

3-(3,4-dichlorophenyl)-1,1-dimethylurea

TES

N-tris(hydroxymethyl)-2-aminoethane-sulfonic acid

TCA

trichloroacetic acid

Tris

N-tris(hydroxymethyl)aminoethane

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Copyright information

© Springer-Verlag 1988

Authors and Affiliations

  • Teresa Thiel
    • 1
  1. 1.Department of BiologyUniversity of Missouri-St. LouisSt. LouisUSA

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