Summary
Hyphae from mixed cultures of complementary auxotrophs of Streptomyces coelicolor A3(2) did not grow on minimal media (MM) when fertility plasmids (SCP1 and SCP2) were missing in both strains. The addition of one part per cent of complete medium (CM) to MM allowed growth of vigorous colonies among the tiny colonies of the parental types. The former, amounting to 1%–10% of the total population, turned out to be heterokaryons. They could be propagated on the same medium by plating of hyphal fragments. When five parts per cent of CM were added to MM, beside the heterokaryotic colonies vigorous ‘spindles’ of aerial mycelium were formed whenever complementary colonies overlapped. When the SCP1 and SCP2 plasmids were present in one or both parents a clear constraint on the outburst of heterokaryotic aerial mycelium was observed.
References
Bibb MJ, Freeman RG, Hopwood DA (1977) Physical and genetical characterisation of a second sex factor, SCP2, for Streptomyces coelicolor A3(2). Mol Gen Genet 154:155–166
Bibb MJ, Hopwood DA (1981) Genetic studies of the fertility plasmid SCP2 and its SCP2* variants in Streptomyces coelicolor A3(2). J gen Microbiol 126:427–442
Bradley SG (1962) Parasexual phenomena in microorganisms. Ann Rev Microbiol 16:35
Bradley SG, Lederberg W (1959) Heterokaryosis in Streptomyces. J Bacteriol 72:219–225
Braendle DM, Szybalski W, (1959) Heterokaryotic compatibility metabolic cooperation and genic recombination in Strepto myces. Ann N Y Acad Sci 81:824–853
Chater KF, Hopwood DA (1982) Streptomyces genetics. In: Goodfellow M, Mordasky M, Williams ST (eds) Biology of the actinomycetes. Academic Press, London
Fratello B, Khoudokormoff VA, Russi-Polito S (1964) Controllo genetico della biosintesi dell'istidina in Streptomyces coelicolor. Atti Ass Genet It. Pavia 9:71
Hopwood DA (1967) Genetic analysis and genome structure in Streptomyces coelicolor. Bacteriol. Rev 31:373–403
Hopwood DA, Sermonti G (1962) The genetics of Streptomyces coelicolor. Adv Genet 11:273–342
Pontecorvo G (1946) Genetic systems based on heterokaryosis. Cold Spring Harbor Symp Quant Biol 11:193–201
Sermonti G (1969) Genetics of antibiotic producing microorganisms. Wiley Interscience, London
Sermonti G, Bandiera M, Spada-Sermonti I (1966) New approach to the genetics of Streptomyces coelicolor. J Bacteriol 91:384–392
Sermonti G, Lanfaloni L, Micheli MR (1983) Properties of transposon SCTn1 of Streptomyces coelicolor A3(2). Mol Gen Genet 191:158–161
Vivan A (1971) Genetic control of fertility in Streptomyces coelicolor A3(2): plasmid involvement in the interconversion of UF and IF strains. J Gen Microbiol 69:353–364
Author information
Authors and Affiliations
Additional information
Communicated by F. Kaudewitz
Rights and permissions
About this article
Cite this article
Micheli, M., Sermonti, G. & Lanfaloni, L. Evidence of heterokaryosis in Streptomyces coelicolor A3(2). Molec. Gen. Genet. 194, 541–544 (1984). https://doi.org/10.1007/BF00425573
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00425573