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Molecular and General Genetics MGG

, Volume 194, Issue 3, pp 517–522 | Cite as

Simultaneous transposition of different mobile elements: Relation to multiple mutagenesis in Drosophila melanogaster

  • Tatiana I. Gerasimova
  • Liliya V. Matyunina
  • Yurii V. Ilyin
  • Georgii P. Georgiev
Article

Summary

Several different transposition events occur simultaneously in one and the same germ cell, as we have found by analyzing different genetic systems in Drosophila melanogaster. (i) In unstable ctMR2 strains, stable reversions to ct+ and changes in the type of ct mutation, which depend on an excision or transposition of the mobile element mdg4 (Gerasimova 1981; Gerasimova et al. 1984), are frequently accompanied by the appearance of novel mutations in different loci of the X chromosome. Some of these (sn, w, g) seem to be induced by the P-element and copia. (ii) A stable ctMR2 reversion to the wild type frequently coexists with an insertion of one to five copies of the P-element in the X-chromosome. Thus, the number of independent transposition events registered by genetic analysis and in situ hybridization may be as great as six. (iii) In two strains with double unstable mutations (cm, ct, and ct, r), double reversions to the wild type occurred at a high rate (80%–97% of total revertants). They frequently coexisted with novel strain-specific mutations. (iv) The stable strain ct6 g2is destabilized by crossing with the MRh12/Cy strain (which contains a number of P-element copies). Both mutations begin to revert to the wild type. Of the revertants 50% have double reversions. Our experiments revealed a high specificity of insertion sites depending on the nature of transposon and the strain genotype. A possible role played by the burst of transposition in the evolution and possible mechanisms of transposition specificity are discussed.

Keywords

Genetic Analysis Germ Cell High Specificity Insertion Site Mobile Element 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Ananiev EV, Gvozdev VA, Ilyin YV, Tchurikov NA, Georgiev GP (1978) Reiterated genes with varying location in intercalary heterochromatin regions of Drosophila melanogaster polytene chromosomes. Chromosoma 65:359–371Google Scholar
  2. Bingham PM, Kidwell MC, Rubin GM (1982) The molecular basis of P-M hybrid dysgenesis: the role of the P-element, a P-strain specific transposon family. Cell 29:995–1003Google Scholar
  3. Evgen'ev MB, Yenikolopov GN, Peunova NI, Ilyin YV (1982) Transposition of mobile genetic elements in interspecific hybrids of Drosophila. Chromosoma 85:375–386Google Scholar
  4. Gerasimova TI (1981) Genetic instability at the cut locus of Drosophila melanogaster induced by the MRh12 chromosome. Mol Gen Genet 184:544–547Google Scholar
  5. Gerasimova TI (1983a) Superunstable alleles at the cut locus in Drosophila melanogaster. Mol Gen Genet 190:390–393.Google Scholar
  6. Gerasimova TI (1983b) Simultaneous reversions of two unstable alleles at carmine and cut loci in D. melanogaster. Drosophila Information. Service 59:38Google Scholar
  7. Gerasimova TI, Ilyin YV, Mizrokhi LJ, Semjonova LV, Georgiev GP (1984) Mobilization of the transposable element mdg4 by hybrid dysgenesis generates a family of unstable cut mutations in Drosophila melanogaster. Mol Gen Genet (in press)Google Scholar
  8. Golubovsky MD, Zakharov IK (1979) Simultaneous reversion of two unstable mutations in the X-chromosome of D. melanogaster. Genetika (Russ) 15:1599–1609Google Scholar
  9. Golubovsky MD, Ivanov YN, Zakharov IK, Berg RL (1974) Investigation of synchronous and similar changes of the gene pool in geographically separated natural populations of Drosophila melanogaster. Genetika (Russ) 10:72–83Google Scholar
  10. Green MM (1977) Genetic instability in Drosophila melanogaster: de novo induction of putative insertion mutations. Proc Natl Acad Sci USA 74:3490–3493Google Scholar
  11. Kidwell MG, Kidwell TA, Sved TA (1977) Hybrid dysgenesis in Drosophila melanogaster: a syndrome of abberant traits including mutation sterility, male recombination. Genetics 86:813–833Google Scholar
  12. Leigh Brown AJ (1983) Variations at the 87A heat shock locus in Drosophila melanogaster. Proc Natl Acad Sci USA 80:5330–5333Google Scholar
  13. Lindsley DL and Grell EH (1968) Genetic variations of Drosophila melanogaster. Carnegie Inst Wash Publ No 627Google Scholar
  14. Modolell J, Bender W, Meselson M (1983) Drosophila melanogaster mutations supressible by the supressor Hairy-wing are insertions of a 7.3 kilobase mobile element. Proc Natl Acad Sci USA 80:1678–1682Google Scholar
  15. Rigby PWS, Dieckmann M, Rhodes C, Berg P (1977) Labelling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol 113:237–251Google Scholar
  16. Rubin GM, Kidwell MG, Bingham PM (1982) The molecular basis of P-M hybrid dysgenesis: the nature of induced mutations. Cell 29:987–994Google Scholar
  17. Rubin GM, Spradling AC (1982) Genetic transformation of Drosophila with transposable element vectors. Science 218:348–353Google Scholar
  18. Tchurikov NA, Ilyin YV, Skryabin KG, Ananiev EV, Bayev AA, Krayev AS, Zelentsova ES, Kulguskin VV, Lyubomirskaya NV, Georgiev GP (1981) General properties of mobile dispersed genetic elements in Drosophila melanogaster. Cold Spring Harbor Symp Quant Biol 45:655–665Google Scholar

Copyright information

© Springer-Verlag 1984

Authors and Affiliations

  • Tatiana I. Gerasimova
    • 1
  • Liliya V. Matyunina
    • 1
  • Yurii V. Ilyin
    • 2
  • Georgii P. Georgiev
    • 2
  1. 1.Institute of Molecular GeneticsUSSR Academy of SciencesMoscowUSSR
  2. 2.Institute of Molecular BiologyUSSR Academy of SciencesMoscowUSSR

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