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Location by DNA sequence analysis of cop mutations affecting the number of plasmid copies of prophage P1

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Summary

The DNA sequence of six P1 cop mutants, which are altered in the control of copy number of the plasmid prophage, was compared to that of P1 wild type. Each cop mutant differs from the wild type by a single base substitution. All of these substitutions are located within a 400 base pair region of P1 DNA that also encodes rep, a gene whose product is required for P1 replication.

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References

  • Abeles AL, Snyder KM, Chattoraj DK (1984) J Mol Biol (in press)

  • Alton NK, Vapnek D (1978) Molecular cloning of restriction fragments and construction of a physical and genetic map of E. coli plasmid R538-1. Plasmid 1:388–404

    Google Scholar 

  • Bächi B, Arber W (1977) Physical mapping of Bg/II, BamHI, EcoRI, HindIII, and PstI restriction fragments of bacteriophage P1 DNA. Mol Gen Genet 153:311–324

    Google Scholar 

  • Bethesda Research Laboratories (1980) M13 cloning/“dideoxy” sequencing manual. Gaithersburg, MD, USA

  • Cesarini G (1981) The target of the negative regulator of pMB1 replication overlaps with part of the repressor coding sequence. Mol Gen Genet 184:40–45

    Google Scholar 

  • Collins J, Pritchard R (1973) Relationship between chromosome replication and F'lac episome replication in Escherichia coli. J Mol Biol 78:143–155

    Google Scholar 

  • Cowan J, Scott JR (1981) Incompatibility among group Y plasmids. Plasmid 6:202–221

    Google Scholar 

  • Danbara H, Brady G, Timmis JK, Timmis KN (1981) Regulation of DNA replication: “target” determinant of the replication control elements of plasmid R6-5 lies within a control element gene. Proc Natl Acad Sci USA 78:4699–4703

    Google Scholar 

  • Froehlich BJ, Tatti K, Scott JR (1983) Evidence for positive regulation of replication of the plasmid prophage P1: Integrative suppression by copy mutants. J Bacteriol 156:205–211

    Google Scholar 

  • Itoh T, Tomizawa J (1980) Formation of an RNA primer for initilation of replication of ColE1 DNA by ribonuclease H. Proc Natl Acad Sci USA 77:2450–2454

    Google Scholar 

  • Mural RJ, Chesney RH, Vapnek D, Kropf MM, Scott JR (1979) Isolation and characterization of cloned fragments of bacteriophage P1 DNA. Virology 93:387–397

    Google Scholar 

  • Murotsu T, Matsubara K, Sugisaki H, Takanami M (1981) Nine unique repeating sequences in a region essential for replication and incompatibility of the mini-F plasmid. Gene 15:257–271

    Google Scholar 

  • Nordstrom K (1983) Control of plasmid replication: a synthesis occasioned by the recent EMBO workshop “Replication of prokaryotic DNA”, held at Eemhof, The Netherlands, May, 1982. Plasmid 9:1–7

    Google Scholar 

  • Prentki P, Chandler M, Caro L (1977) Replication of the prophage P1 during the cell cycle of Escherichia coli. Mol Gen Genet 152:71–76

    Google Scholar 

  • Pritchard RH, Barth PT, Collins J (1969) Control of DNA synthesis is bacteria, Symp Soc Gen Microbiol 19:263–297

    Google Scholar 

  • Sanger F, Nicklen S, Coulson AR (1977) DNA sequencing with chain terminating inhibitors. Proc Natl Acad Sci USA 74:5463–5467

    Google Scholar 

  • Scott JR (1984) Regulation of plasmid replication. Microbiol Rev (in press)

  • Scott JR, Kropf MM, Padolski L, Goodspeed JK, David R, Vapnek D (1982) Mutants of plasmid prophage P1 with elevated copy number: Isolation and characterization. J Bacteriol 150:1329–1339

    Google Scholar 

  • Sogaard-Andersen L, Rokeach LA, Molin S (1983) Regulated expression of a gene important for replication of plasmid F in E. coli (submitted)

  • Sompayrac L, Maaloe O (1973) Autorepressor model for control of DNA replication. Nature New Biol 241:133–135

    Google Scholar 

  • Stougaard P, Molin S, Nordstrom K (1981) RNAs involved in copy number control and incompatibility of plasmid R1. Proc Natl Acad Sci USA 78:6608–6612

    Google Scholar 

  • Stougaard P, Light J, Molin S (1982) Convergent transcription interferes with expression of the copy number control gene copA, from plasmid R1. EMBO J 1:323–328

    Google Scholar 

  • Tomizawa J, Itoh T (1981) Plasmid ColE1 incompatibility determined by interaction of RNAI with primer transcript. Proc Natl Acad Sci USA 78:6096–6100

    Google Scholar 

  • Vieira J, Messing T (1982) The pUC plasmid, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. Gene 19:259–268

    Google Scholar 

  • Yarmolinsky M (1982) Bacteriophage P1. In: O'Brien SJ (ed) Genetic maps, vol 2. pp 34–43

  • Yun T, Vapnek D (1977) Electron microscopic analysis of bacteriophage, P1, phage P1Cm, and phage P7. Determination of genome sizes, sequence homology and location of antibiotic resistance determinants. Virology 77:376–385

    Google Scholar 

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Communicated by W. Arber

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Baumstark, B.R., Lowery, K. & Scott, J.R. Location by DNA sequence analysis of cop mutations affecting the number of plasmid copies of prophage P1. Molec. Gen. Genet. 194, 513–516 (1984). https://doi.org/10.1007/BF00425567

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  • DOI: https://doi.org/10.1007/BF00425567

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