Summary
The possibility of cloning filamentous fungal genes by expression in the yeast Saccharomyces cerevisiae has been studied. A genome bank of Aspergillus niger was made in E. coli using a yeast cosmid shuttle vector and over 10,000 different cosmid clones were individually isolated. Yeast transformants carrying Aspergillus DNA were screened for the expression of the genes for fungal secreted glycoproteins, β-galactosidase, β-glucosidase, and amyloglucosidase, and for the expression of fungal genes complementing yeast ura3 and leu2 mutations.
Of the five Aspergillus genes studied, only one, β-glucosidase, was found to be expressed in yeast, and this at a low level. This suggests that there are essential differences between the genes of yeast and filamentous fungi.
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Communicated by Ch. Auerbach
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Penttilä, M.E., Helena Nevalainen, K.M., Raynal, A. et al. Cloning of Aspergillus niger genes in yeast. Expression of the gene coding Aspergillus β-glucosidase. Molec. Gen. Genet. 194, 494–499 (1984). https://doi.org/10.1007/BF00425564
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DOI: https://doi.org/10.1007/BF00425564